Biomimetic porous high-density polyethylene/polyethylene-grafted-maleic anhydride scaffold with improved in vitro cytocompatibility

Sharma, S and Bhaskar, N and Bose, S and Basu, B (2018) Biomimetic porous high-density polyethylene/polyethylene-grafted-maleic anhydride scaffold with improved in vitro cytocompatibility. In: Journal of Biomaterials Applications, 32 (10). pp. 1450-1463.

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Abstract

A major challenge for tissue engineering is to design and to develop a porous biocompatible scaffold, which can mimic the properties of natural tissue. As a first step towards this endeavour, we here demonstrate a distinct methodology in biomimetically synthesized porous high-density polyethylene scaffolds. Co-extrusion approach was adopted, whereby high-density polyethylene was melt mixed with polyethylene oxide to form an immiscible binary blend. Selective dissolution of polyethylene oxide from the biphasic system revealed droplet–matrix-type morphology. An attempt to stabilize such morphology against thermal and shear effects was made by the addition of polyethylene-grafted-maleic anhydride as a compatibilizer. A maximum ultimate tensile strength of 7 MPa and elastic modulus of 370 MPa were displayed by the high-density polyethylene/polyethylene oxide binary blend with 5% maleated polyethylene during uniaxial tensile loading. The cell culture experiments with murine myoblast C2C12 cell line indicated that compared to neat high-density polyethylene and high-density polyethylene/polyethylene oxide, the high-density polyethylene/polyethylene oxide with 5% polyethylene-grafted-maleic anhydride scaffold significantly increased muscle cell attachment and proliferation with distinct elongated threadlike appearance and highly stained nuclei, in vitro. This has been partly attributed to the change in surface wettability property with a reduced contact angle (∼72°) for 5% PE-g-MA blends. These findings suggest that the high-density polyethylene/polyethylene oxide with 5% polyethylene-grafted-maleic anhydride can be treated as a cell growth substrate in bioengineering applications.

Item Type:	Journal Article
Publication:	Journal of Biomaterials Applications
Publisher:	SAGE Publications Ltd
Additional Information:	The copyright for this article belongs to the SAGE Publications Ltd.
Keywords:	Biocompatibility; Biomimetics; Cell culture; Cells; Compatibilizers; High density polyethylenes; Maleic anhydride; Polyethylene oxides; Scaffolds; Scaffolds (biology); Shear flow; Tensile strength; Tissue, Biocompatible scaffolds; Cytocompatibility; Immiscible binary blends; Maleated polyethylenes; Selective dissolution; Surface wettability; Ultimate tensile strength; Uniaxial tensile loading, Polyethylenes, macrogol; maleic anhydride; polyethylene; biomaterial; biomimetic material; maleic anhydride; polyethylene, animal cell; Article; biocompatibility; biomimetics; C2C12 cell line; cell adhesion; cell growth; cell proliferation; cell spreading; contact angle; controlled study; crystallization; dissolution; in vitro study; mouse; nonhuman; porosity; priority journal; surface property; tensile strength; thermostability; wettability; Young modulus; animal; cell line; chemistry; flow kinetics; materials testing; tissue scaffold, Animals; Biocompatible Materials; Biomimetic Materials; Cell Adhesion; Cell Line; Cell Proliferation; Maleic Anhydrides; Materials Testing; Mice; Polyethylene; Porosity; Rheology; Tensile Strength; Tissue Scaffolds
Department/Centre:	Division of Chemical Sciences > Materials Research Centre Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited:	08 Aug 2022 06:24
Last Modified:	08 Aug 2022 06:24
URI:	https://eprints.iisc.ac.in/id/eprint/75567

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