Serine/threonine/tyrosine-interacting-like protein 1 (STYXL1), a pseudo phosphatase, promotes oncogenesis in glioma

Tomar, Vivek Singh and Baral, Tapan Kumar and Nagavelu, Krishnaveni and Somasundaram, Kumaravel (2019) Serine/threonine/tyrosine-interacting-like protein 1 (STYXL1), a pseudo phosphatase, promotes oncogenesis in glioma. In: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 515 (1). pp. 241-247.

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Abstract

Phosphatases play an important role in cellular signaling and are often found dysregulated in cancers including glioblastoma (GBM). A comprehensive bioinformatics analysis of phosphatases (n = 403) in multiple datasets revealed their deregulation in GBM. Among the differentially regulated phosphatases (n = 186; 46.1%), majority of them were found to be regulated by microRNA (n = 94; 50.5%) followed by DNA methylation (n = 22; 11.8%) and altered copy number variation (n = 10; 5.37%). STYXLI (Serine/threonine/tyrosine-interacting-like protein 1) was found to be the second most amplified gene in GBM, upregulated, and correlated to poor prognosis. The expression of STYXL1 was also found to be higher in IDH1 mutant gliomas and G-CIMP- gliomas which are reported to be more aggressive than their corresponding counterparts. Silencing STYXLI inhibited glioma cell growth, soft agar colony formation, migration, invasion, proliferation, and xenograft tumor growth. Further, ectopic expression of STYXLI was found to promote glioma cell growth, soft agar colony formation, migration, and RasV12 induced in-vitro transformation of immortalized human astrocytes, thus confirming its oncogenic potential in GBM. In this report, we provide a comprehensive overview of deregulation of phosphatases in GBM and demonstrate for the first time, the oncogenic nature of STYXLI in GBM. This study might be useful for treatment of GBM patients with deregulated STYXLI.

Item Type:	Journal Article
Publication:	BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Publisher:	ACADEMIC PRESS INC ELSEVIER SCIENCE
Additional Information:	copyright for this article belongs to ACADEMIC PRESS INC ELSEVIER SCIENCE
Keywords:	Glioblastoma; Phosphatases; Regulation
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology Division of Biological Sciences > Central Animal Facility (Formerly Primate Research Laboratory)
Date Deposited:	13 Sep 2019 10:42
Last Modified:	13 Sep 2019 10:42
URI:	http://eprints.iisc.ac.in/id/eprint/63187

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