Visible light-based 3D bioprinted composite scaffolds of κ-carrageenan for bone tissue engineering applications

Kumari, S and Mondal, P and Tyeb, S and Chatterjee, K (2024) Visible light-based 3D bioprinted composite scaffolds of κ-carrageenan for bone tissue engineering applications. In: Journal of Materials Chemistry B .

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Abstract

Three-dimensional (3D) printing of bone scaffolds using digital light processing (DLP) bioprinting technology empowers the treatment of patients suffering from bone disorders and defects through the fabrication of cell-laden patient-specific scaffolds. Here, we demonstrate the visible-light-induced photo-crosslinking of methacrylate-κ-carrageenan (MA-κ-CA) mixed with bioactive silica nanoparticles (BSNPs) to fabricate 3D composite hydrogels using digital light processing (DLP) printing. The 3D printing of complex bone structures, such as the gyroid, was demonstrated with high precision and resolution. DLP-printed 3D composite hydrogels of MA-κ-CA-BSNP were prepared and systematically assessed for their macroporous structure, swelling, and degradation characteristics. The viscosity, rheological, and mechanical properties were also investigated for the influence of nanoparticle incorporation in the MA-κ-CA hydrogels. The in vitro study performed with MC3T3-E1 pre-osteoblast-laden scaffolds of MA-κ-CA-BSNP revealed high cell viability, no cytotoxicity, and proliferation over 21 days with markedly enhanced osteogenic differentiation compared to neat polymeric scaffolds. Furthermore, no inflammation was observed in the 21-day study involving the in vivo examination of DLP-printed 3D composite scaffolds in a Wistar rat model. Overall, the observed results for the DLP-printed 3D composite scaffolds of MA-κ-CA and BSNP demonstrate their biocompatibility and suitability for bone tissue engineering. © 2024 The Royal Society of Chemistry.

Item Type:	Journal Article
Publication:	Journal of Materials Chemistry B
Publisher:	Royal Society of Chemistry
Additional Information:	The copyright for this article belongs to Royal Society of Chemistry.
Keywords:	3D printing; Biocompatibility; Bone; Hydrogels; Patient treatment; Scaffolds (biology); Silica nanoparticles, 3D composites; Bioactives; Bone tissue engineering; Carrageenans; Composite hydrogels; Composite scaffolds; Digital light processing; Silica nanoparticles; Tissue engineering applications; Visible light, Biomechanics
Department/Centre:	Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited:	04 Mar 2024 07:04
Last Modified:	04 Mar 2024 07:04
URI:	https://eprints.iisc.ac.in/id/eprint/84186

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