Kanojia, A and Sharma, M and Shiraz, R and Tripathi, S (2022) Flavivirus–Host Interaction Landscape Visualized through Genome-Wide CRISPR Screens. In: Viruses, 14 (10).
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Abstract
Flaviviruses comprise several important human pathogens which cause significant morbidity and mortality worldwide. Like any other virus, they are obligate intracellular parasites. Therefore, studying the host cellular factors that promote or restrict their replication and pathogenesis becomes vital. Since inhibiting the host dependency factors or activating the host restriction factors can suppress the viral replication and propagation in the cell, identifying them reveals potential targets for antiviral therapeutics. Clustered regularly interspaced short palindromic repeats (CRISPR) technology has provided an effective means of producing customizable genetic modifications and performing forward genetic screens in a broad spectrum of cell types and organisms. The ease, rapidity, and high reproducibility of CRISPR technology have made it an excellent tool for carrying out genome-wide screens to identify and characterize viral host dependency factors systematically. Here, we review the insights from various Genome-wide CRISPR screens that have advanced our understanding of Flavivirus-Host interactions. © 2022 by the authors.
| Item Type: | Journal Article |
|---|---|
| Publication: | Viruses |
| Publisher: | MDPI |
| Additional Information: | The copyright for this article belongs to MDPI. |
| Keywords: | antivirus agent, clustered regularly interspaced short palindromic repeat; CRISPR Cas system; Flavivirus; genetics; human; reproducibility; virus, Antiviral Agents; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR-Cas Systems; Flavivirus; Humans; Reproducibility of Results; Viruses |
| Department/Centre: | Division of Biological Sciences > Microbiology & Cell Biology Division of Biological Sciences > Centre for Infectious Disease Research |
| Date Deposited: | 29 Nov 2022 09:09 |
| Last Modified: | 29 Nov 2022 09:09 |
| URI: | https://eprints.iisc.ac.in/id/eprint/77894 |
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