George, E and Goswami, A and Lodhiya, T and Padwal, P and Iyer, S and Gauttam, I and Sethi, L and Jeyasankar, S and Sharma, PR and Dravid, AA and Mukherjee, R and Agarwal, R (2022) Immunomodulatory effect of mycobacterial outer membrane vesicles coated nanoparticles. In: Biomaterials Advances, 139 .
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Abstract
Tuberculosis (TB) is one of the most widely prevalent infectious diseases that cause significant mortality. Bacillus Calmette-Guérin (BCG), the current TB vaccine used in clinics, shows variable efficacy and has safety concerns for immunocompromised patients. There is a need to develop new and more effective TB vaccines. Outer membrane vesicles (OMVs) are vesicles released by Mycobacteria that contain several lipids and membrane proteins and act as a good source of antigens to prime immune response. However, the use of OMVs as vaccines has been hampered by their heterogeneous size and low stability. Here we report that mycobacterial OMVs can be stabilized by coating over uniform-sized 50 nm gold nanoparticles. The OMV-coated gold nanoparticles (OMV-AuNP) show enhanced uptake and activation of macrophages and dendritic cells. Proteinase K and TLR inhibitor studies demonstrated that the enhanced activation was attributed to proteins present on OMVs and was mediated primarily by TLR2 and TLR4. Mass spectrometry analysis revealed several potential membrane proteins that were common in both free OMVs and OMV-AuNP. Such strategies may open up new avenues and the utilization of novel antigens for developing TB vaccines.
| Item Type: | Journal Article |
|---|---|
| Publication: | Biomaterials Advances |
| Publisher: | Elsevier Ltd |
| Additional Information: | The copyright for this article belongs to the Authors. |
| Keywords: | Antigens; Bacteriology; Chemical activation; Diseases; Fiber optic sensors; Mass spectrometry; Metal nanoparticles; Proteins; Vaccines, Coated nanoparticles; Exosomes; Gold nanoparticle; Gold Nanoparticles; Immunomodulatory effects; Infectious disease; Membrane proteins; Mycobacterial; Outer membrane vesicles; Tuberculosis, Gold nanoparticles |
| Department/Centre: | Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering |
| Date Deposited: | 20 Jul 2022 11:14 |
| Last Modified: | 20 Jul 2022 11:14 |
| URI: | https://eprints.iisc.ac.in/id/eprint/74936 |
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