Lysinibacillus macroides-mediated control of cellulose-producing morphotype of Salmonella

Karmakar, K and Bhattacharya, R and Sharma, A and Parmar, K and Nath, U and Nataraja, KN and Earanna, N and Sharma, G and Chakravortty, D (2022) Lysinibacillus macroides-mediated control of cellulose-producing morphotype of Salmonella. In: Journal of the Science of Food and Agriculture .

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Abstract

BACKGROUND: Soil-dwelling human pathogens like Salmonella are transmitted by fresh produce such as tomato, spinach, onion and cabbage. With >2600 serovars, it is difficult to classify the good plant colonizers from the non-colonizers. Generally, soil microbiota are classified as autochthonous or zymogenous organisms, based on their ability to survive in soil. However, such information for soil-dwelling human pathogens is not available Thus there is a need to classify these organisms for designing a strategy to prevent their outbreak. Moreover, soil harbours a plethora of microbes, which can be screened for competitive organisms to control such human pathogens. RESULTS: In this study, we examined whether the morphotype based on the attachment factors (e.g., cellulose and curli fimbri) of Salmonella was important for its colonization of roots. Secondly, we tracked the location of the bacteria in the plant cell. Interestingly, most of the epidermal cells occupied by Salmonella showed propidium iodide-positive nuclei. As an extension of the study, a screening of competitive rhizospheric bacteria was performed. One isolate, identified as Lysinibacillus macroides, was able to inhibit the biofilm of Salmonella and subsequently reduced its colonization on roots. CONCLUSION: Based on this study, we classified the Rdar (red, dry and rough) morphotypes as good plant colonists. The ability to colonize and subsequent kill the live plant cell throws light on the zymogenous life cycle of soil-dwelling Salmonella. Additionally, Lysinibacillus macroides served as a biocontrol agent by reducing the burden of Salmonella in various vegetables. Such organisms can further be explored to prevent contamination of the food chain. © 2022 Society of Chemical Industry.

Item Type:	Journal Article
Publication:	Journal of the Science of Food and Agriculture
Publisher:	John Wiley and Sons Ltd
Additional Information:	The copyright for this article belongs to the Society of Chemical Industry, John Wiley and Sons Ltd
Keywords:	cellulose; Lysinibacillus; morphotype; propidium iodide; Salmonella; serovar
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	04 Jul 2022 06:12
Last Modified:	04 Jul 2022 06:12
URI:	https://eprints.iisc.ac.in/id/eprint/74154

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