Yadav, P and Shah, R and Roy, A and Jani, S and Chatterjee, K and Saini, DK (2023) Cellular Senescence Program is Sensitive to Physical Differences in Polymeric Tissue Scaffolds. In: ACS Materials Au .
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Abstract
A typical cellular senescence program involves exposing cells to DNA-damaging agents such as ionization radiation or chemotherapeutic drugs, which cause multipronged changes, including increased cell size and volume, the onset of enhanced oxidative stress, and inflammation. In the present study, we examined if the senescence onset decision is sensitive to the design, porosity, and architecture of the substrate. To address this, we generated a library of polymeric scaffolds widely used in tissue engineering of varied stiffness, architecture, and porosity. Using irradiated A549 lung cancer cells, we examined the differences between cellular responses in these 3D scaffold systems and observed that senescence onset is equally diminished. When compared to the two-dimensional (2D) culture formats, there were profound changes in cell size and senescence induction in three-dimensional (3D) scaffolds. We further establish that these observed differences in the senescence state can be attributed to the altered cell spreading and cellular interactions on these substrates. This study elucidates the role of scaffold architecture in the cellular senescence program. © 2023 The Authors. Published by American Chemical Society.
| Item Type: | Journal Article |
|---|---|
| Publication: | ACS Materials Au |
| Publisher: | American Chemical Society |
| Additional Information: | The copyright for this article belongs to author. |
| Department/Centre: | Division of Biological Sciences > Molecular Reproduction, Development & Genetics Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy) |
| Date Deposited: | 04 Mar 2024 09:32 |
| Last Modified: | 04 Mar 2024 09:32 |
| URI: | https://eprints.iisc.ac.in/id/eprint/84350 |
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