Modulation of protein-graphene oxide interactions with varying degrees of oxidation

Malik, SA and Mohanta, Z and Srivastava, C and Atreya, HS (2020) Modulation of protein-graphene oxide interactions with varying degrees of oxidation. In: Nanoscale Advances, 2 (5). pp. 1904-1912.

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Abstract

The degree of oxidation of graphene oxide (GO) has been shown to be important for its toxicity and drug-loading efficiency. However, the effect of its variations on GO-protein interaction remains unclear. Here, we evaluate the effect of the different oxidation degrees of GO on its interaction with human ubiquitin (8.6 kDa) using solution state nuclear magnetic resonance (NMR) spectroscopy in combination with other biophysical techniques. Our findings show that the interaction between the protein and the different GO samples is weak and electrostatic in nature. It involves fast dynamic exchange of the protein molecules from the surface of the GO. As the oxidation degree of the GO increases, the extent of the interaction with the protein changes. The interaction of the protein with GO can thus be modulated by tuning the degree of oxidation. This study opens up new avenues to design appropriate graphenic materials for use in various biomedical fields such as drug delivery, biomedical devices and imaging. © The Royal Society of Chemistry 2020.

Item Type:	Journal Article
Publication:	Nanoscale Advances
Additional Information:	all the copyrights belongs to authors.
Keywords:	Drug delivery; Graphene; Nuclear magnetic resonance; Nuclear magnetic resonance spectroscopy; Oxidation, Biomedical devices; Biomedical fields; Biophysical techniques; Degree of oxidations; Drug-loading efficiency; Protein interaction; Protein molecules; Solution state nuclear magnetic resonance, Proteins
Department/Centre:	Division of Chemical Sciences > Solid State & Structural Chemistry Unit Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy) Others
Date Deposited:	16 Dec 2024 12:21
Last Modified:	16 Dec 2024 12:21
URI:	http://eprints.iisc.ac.in/id/eprint/65860

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