Emerging technologies for antibiotic susceptibility testing

Behera, Bhagaban and Vishnu, Anil G K and Chatterjee, Suman and Sitaramgupta, V S N V and Sreekumar, Niranjana and Nagabhushan, Apoorva and Rajendran, Nirmala and Prathik, B H and Pandya, Hardik J (2019) Emerging technologies for antibiotic susceptibility testing. In: BIOSENSORS & BIOELECTRONICS, 142 .

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Abstract

Superbugs such as infectious bacteria pose a great threat to humanity due to an increase in bacterial mortality leading to clinical treatment failure, lengthy hospital stay, intravenous therapy and accretion of bacteraemia. These disease-causing bacteria gain resistance to drugs over time which further complicates the treatment. Monitoring of antibiotic resistance is therefore necessary so that bacterial infectious diseases can be diagnosed rapidly. Antimicrobial susceptibility testing (AST) provides valuable information on the efficacy of antibiotic agents and their dosages for treatment against bacterial infections. In clinical laboratories, most widely used AST methods are disk diffusion, gradient diffusion, broth dilution, or commercially available semi-automated systems. Though these methods are cost-effective and accurate, they are time-consuming, labour-intensive, and require skilled manpower. Recently much attention has been on developing rapid AST techniques to avoid misuse of antibiotics and provide effective treatment. In this review, we have discussed emerging engineering AST techniques with special emphasis on phenotypic AST. These techniques include fluorescence imaging along with computational image processing, surface plasmon resonance, Raman spectra, and laser tweezer as well as micro/nanotechnology-based device such as microfluidics, microdroplets, and microchamber. The mechanical and electrical behaviour of single bacterial cell and bacterial suspension for the study of AST is also discussed.

Item Type:	Journal Article
Publication:	BIOSENSORS & BIOELECTRONICS
Publisher:	ELSEVIER ADVANCED TECHNOLOGY
Additional Information:	copyright for this article belongs to ELSEVIER ADVANCED TECHNOLOGY
Keywords:	Bacteria; Antibiotics; AST; Emerging technologies; Microfabrication
Department/Centre:	Division of Electrical Sciences > Electronic Systems Engineering (Formerly Centre for Electronic Design & Technology) Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering Others
Date Deposited:	24 Oct 2019 05:29
Last Modified:	24 Oct 2019 05:29
URI:	http://eprints.iisc.ac.in/id/eprint/63761

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