Molecular determinants of complex formation between DNA and the AT-rich interaction domain of BAF250a

Maulik, Aditi and Giri, Malyasree and Singh, Mahavir (2019) Molecular determinants of complex formation between DNA and the AT-rich interaction domain of BAF250a. In: FEBS LETTERS, 593 (19). pp. 2716-2729.

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Abstract

AT-rich interaction domain (ARID)-containing BAF250a protein is a central DNA-binding subunit of the SWI/SNF chromatin-remodeling complex. ARIDs are found in several eukaryotic proteins that play roles in different aspects of cellular physiology. However, despite their biological importance, ARIDs remain relatively uncharacterized for their dynamics and DNA binding. Here, we have probed the structure and DNA-binding properties of BAF250a ARID. We show that the core BAF250a ARID interacts with DNA sequences with low micromolar affinities. NMR chemical shift perturbation (CSP) results reveal a number of conserved residues in ARID that are involved in DNA binding. An NMR CSP-based docking model of ARID-DNA complexes reveals that BAF250a ARID possesses necessary determinants of specific DNA binding.

Item Type:	Journal Article
Publication:	FEBS LETTERS
Publisher:	WILEY
Additional Information:	Copyright of this article belongs to WILEY
Keywords:	RID FAMILY; BINDING; PROTEINS; SEQUENCE; CANCER; DYNAMICS; SUBUNITS
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit Division of Chemical Sciences > NMR Research Centre (Formerly Sophisticated Instruments Facility)
Date Deposited:	19 Nov 2019 06:17
Last Modified:	19 Nov 2019 06:17
URI:	http://eprints.iisc.ac.in/id/eprint/63484

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