Overexpression, purification, crystallization and preliminary X-ray analysis of uracil N-glycosylase from Mycobacterium tuberculosis in complex with a proteinaceous inhibitor

Singh, Prem and Talawar, Ramappa K and Krishna, PDV and Varshney, Umesh and Vijayan, M (2006) Overexpression, purification, crystallization and preliminary X-ray analysis of uracil N-glycosylase from Mycobacterium tuberculosis in complex with a proteinaceous inhibitor. In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 62 (12). pp. 1231-1234.

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Abstract

Uracil N-glycosylase is an enzyme which initiates the pathway of uracil-excision repair of DNA. The enzyme from Mycobacterium tuberculosis was co-expressed with a proteinaceous inhibitor from Bacillus subtilis phage and was crystallized in monoclinic space group C2, with unit-cell parameters a = 201.14, b = 64.27, c = 203.68 \AA , $\beta = 109.7^o$. X-ray data from the crystal have been collected for structure analysis.

Item Type:	Journal Article
Publication:	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Publisher:	International Union of Crystallography
Additional Information:	Copyright of this article belongs to International Union of Crystallography.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited:	11 Jan 2007
Last Modified:	19 Sep 2010 04:33
URI:	http://eprints.iisc.ac.in/id/eprint/9272

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