Multiscale vapor-mediated dendritic pattern formation and bacterial aggregation in complex respiratory biofluid droplets

Hegde, O and Chatterjee, R and Rasheed, A and Chakravortty, D and Basu, S (2022) Multiscale vapor-mediated dendritic pattern formation and bacterial aggregation in complex respiratory biofluid droplets. In: Journal of Colloid and Interface Science, 606 . pp. 2011-2023.

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Abstract

Hypothesis: Deposits of biofluid droplets on surfaces (such as respiratory droplets formed during an expiratory) are composed of water-based salt-protein solution that may also contain an infection (bacterial/viral). The final patterns of the deposit formed and bacterial aggregation on the deposits are dictated by the fluid composition and flow dynamics within the droplet. Experiments: This work reports the spatio-temporal, topological regulation of deposits of respiratory fluid droplets and control of bacterial aggregation by tweaking flow inside droplets using non-contact vapor-mediated interactions. Desiccated respiratory droplets form deposits with haphazard multiscale dendritic, cruciform-shaped precipitates when evaporated on a glass substrate. However, we showcase that short and long-range vapor-mediated interaction between the droplets can be used as a tool to control these deposits at nano-micro-millimeter scales. We morphologically control hierarchial dendrite size, orientation and subsequently suppress cruciform-shaped crystals by placing a droplet of ethanol in the vicinity of the biofluid droplet. Active living matter in respiratory fluids like bacteria is preferentially segregated and agglomerated without its viability and pathogenesis attenuation. Findings: The nucleation sites can be controlled via preferential transfer of solutes in the droplets; thus, achieving control over crystal occurrence, growth dynamics, and the final topology of the deposit. For the first time, we have experimentally presented a proof-of-concept to control the aggregation of live active matter like bacteria without any direct contact. The methodology can have ramifications in biomedical applications like disease detection and bacterial segregation. © 2021 Elsevier Inc.

Item Type:	Journal Article
Publication:	Journal of Colloid and Interface Science
Publisher:	Academic Press Inc.
Additional Information:	The copyright for this article belongs to Academic Press Inc.
Keywords:	Agglomeration; Body fluids; Crystal orientation; Deposits; Drops; Medical applications; Self assembly; Substrates; Topology, Biofluid-droplet; Biofluids; Dendritic patterns; Fluid composition; Mediated interaction; Nano-micro crystallization; Pattern formation; Protein solution; Vapor mediated interaction; Water based, Bacteria
Department/Centre:	Division of Biological Sciences > Microbiology & Cell Biology Division of Mechanical Sciences > Mechanical Engineering
Date Deposited:	06 Jan 2022 06:16
Last Modified:	06 Jan 2022 06:16
URI:	http://eprints.iisc.ac.in/id/eprint/70801

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