Helical nanobots as mechanical probes of intra- And extracellular environments

Pal, M and Dasgupta, D and Somalwar, N and Reshma, VR and Tiwari, MK and Teja, D and Narayana, SM and Katke, A and Jayshree, RS and Bhat, R and Saini, DK and Ghosh, A (2020) Helical nanobots as mechanical probes of intra- And extracellular environments. In: Journal of Physics Condensed Matter, 32 (22).

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Abstract

A rheological probe that can measure mechanical properties of biological milieu at well-defined locations with high spatial resolution, on a time scale faster than most biological processes, can further improve our understanding of how living systems operate and behave. Here, we demonstrate nanorobots actively driven in realistic ex vivo biological systems for fast mechanical measurements with high spatial accuracy. In the various demonstrations of magnetic nanobots as mechanical probes, we report the first direct observation of the internalization of probes by a living cell, the accurate measurement of the 'fluid phase' cytoplasmic viscosity of �200 cP for a HeLa cell, demonstration of intracellular measurements in cells derived from human patients; all of which establish the strength of this novel technique for measurements in both intra- and extracellular environments.

Item Type:	Journal Article
Publication:	Journal of Physics Condensed Matter
Publisher:	Institute of Physics Publishing
Additional Information:	The copyright of this article belongs to Institute of Physics Publishing
Keywords:	Biological systems; Biomechanics; Cell proliferation; Lanthanum compounds; Nanorobotics; Nanorobots; Phosphorus compounds; Viscosity measurement, Accurate measurement; Extracellular environments; Extracellular matrices; High spatial resolution; Intra-cellular measurements; Intracellular environments; Mechanical measurements; Microrheology, Probes
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering Division of Physical & Mathematical Sciences > Physics
Date Deposited:	19 Jun 2020 06:39
Last Modified:	19 Jun 2020 06:39
URI:	http://eprints.iisc.ac.in/id/eprint/65043

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