Cleaning carbohydrate impurities from lignin using Pseudomonas fluorescens

Ghosh, Tanushree and Ngo, Tri-Dung and Kumar, Aloke and Ayranci, Cagri and Tang, Tian (2019) Cleaning carbohydrate impurities from lignin using Pseudomonas fluorescens. In: GREEN CHEMISTRY, 21 (7). pp. 1648-1659.

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Abstract

A novel method of cleaning waste lignin biomass by means of selective biodegradation of cellulose and hemicellulose is investigated. This method uses Pseudomonas fluorescens (P. fluorescens), a non-pathogenic bacterium capable of producing cellulolytic enzymes, cleaving polymeric carbohydrates into free sugars and utilizing them as their carbon source. The growth of P. fluorescens on lignin biomass was confirmed by increasing cell count with time and visualized by scanning electron microscopy (SEM). Depletion of free sugars in the growth medium was measured by Technical Association of the Pulp and Paper Industry (TAPPI) methods and by time-of-flight secondary ion mass spectrometry (ToF SIMS), which showed a significant decrease in the carbohydrate content and an increase in the lignin fraction after P. fluorescens biodegradation. The molar mass distribution of lignin before and after the biodegradation of carbohydrate impurities showed no indication of lignin depolymerization. Our results strongly support the biodegradation of carbohydrate impurities from waste lignin by P. fluorescens as an effective, green and eco-friendly process.

Item Type:	Journal Article
Publication:	GREEN CHEMISTRY
Publisher:	ROYAL SOC CHEMISTRY
Additional Information:	The copyright for this article belongs to Royal Society of Chemistry
Department/Centre:	Division of Mechanical Sciences > Mechanical Engineering
Date Deposited:	11 May 2019 06:28
Last Modified:	11 May 2019 06:28
URI:	http://eprints.iisc.ac.in/id/eprint/62606

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