Rv0474 is a copper-responsive transcriptional regulator that negatively regulates expression of RNA polymerase subunit in Mycobacterium tuberculosis

Raghunandanan, Sajith and Ramachandran, Ranjit and Gomez, Roshna Lawrence and Devanarayanan, Sivasankar and Bommakanti, Akhila and Kondapi, Anand Kumar and Varadarajan, Raghavan and Kumar, Ramakrishnan Ajay (2018) Rv0474 is a copper-responsive transcriptional regulator that negatively regulates expression of RNA polymerase subunit in Mycobacterium tuberculosis. In: FEBS JOURNAL, 285 (20). pp. 3849-3869.

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Abstract

We characterize Rv0474, a putative transcriptional regulatory protein of Mycobacterium tuberculosis, which is found to function as a copper-responsive transcriptional regulator at toxic levels of copper. It is an autorepressor, but at elevated levels (10-250 m) of copper ions the repression is relieved resulting in an increase in Rv0474 expression. Copper-bound Rv0474 is recruited to the rpoB promoter leading to its repression resulting in the growth arrest of the bacterium. Mutational analysis showed that the helix-turn-helix and leucine zipper domains of Rv0474 are essential for its binding to Rv0474 and rpoB promoters, respectively. The mechanism of Rv0474-mediated rpoB regulation seems to be operational only in pathogenic mycobacteria that can persist inside the host.

Item Type:	Journal Article
Publication:	FEBS JOURNAL
Publisher:	WILEY
Additional Information:	Copy right for this article belong to WILEY
Keywords:	autoregulation; copper response; Mycobacterium tuberculosis; transcriptional regulator
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	14 Nov 2018 15:11
Last Modified:	14 Nov 2018 15:11
URI:	http://eprints.iisc.ac.in/id/eprint/61064

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