Biodistribution and toxicity evaluation of sesbania mosaic virus nanoparticles in mice

Vardhan, Vishnu GP and Savithri, HS and Murthy, MRN and Hema, M (2016) Biodistribution and toxicity evaluation of sesbania mosaic virus nanoparticles in mice. In: ARCHIVES OF VIROLOGY, 161 (10). pp. 2673-2681.

PDF Arc_Vir_161-10_2673_2016.pdf - Published Version Restricted to Registered users only Download (5MB) | Request a copy

Abstract

Sesbania mosaic virus (SeMV), a 30-nm spherical plant sobemovirus, is suitable for developing functionalized nanoparticles for biomedical applications. However, the in vivo behavior of SeMV and the clinical impact following its delivery via the oral or intravenous route are not known. To address this question, we examined the biodistribution, toxicity and histopathological changes in SeMV treated mice. No toxic effects were observed in mice administered high doses (100 mg and 200 mg per kg body weight orally or 40 mg and 80 mg per kg body weight intravenously) of SeMV, and they were found to be normal. Analysis of fecal sample showed that SeMV was cleared in 16 h when 20 mg of the virus per kg body weight was administered orally. RT-PCR analysis of blood samples showed that SeMV was present up to 72 h in mice inoculated either intravenously (8 mg/kg body weight) or orally (20 mg/kg body weight). Further, SeMV was found to be localized up to 72 h in spleen and liver tissues of intravenously inoculated mice only. Biochemical and hematological parameters were found to be normal at 6 and 72 h after administration of SeMV. Furthermore, no noticeable changes were observed in histological sections of brain, liver, spleen, lungs and kidney tissue samples collected at 6 and 72 h from SeMV administered mice when compared to control mice. Thus, SeMV appears to be a safe and non-toxic platform that can be tailored as a nanocarrier for in vivo biomedical applications.

Item Type:	Journal Article
Publication:	ARCHIVES OF VIROLOGY
Additional Information:	Copy right for this article belongs to the SPRINGER WIEN, SACHSENPLATZ 4-6, PO BOX 89, A-1201 WIEN, AUSTRIA
Department/Centre:	Division of Biological Sciences > Biochemistry Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	03 Dec 2016 05:08
Last Modified:	03 Dec 2016 05:08
URI:	http://eprints.iisc.ac.in/id/eprint/55220

Actions (login required)

View Item