Bovine serum albumin interacts with silver nanoparticles with a ``side on'' or ``end on'' conformation

Dasgupta, Nandita and Ranjan, Shivendu and Patra, Dhabaleswar and Srivastava, Priyanka and Kumar, Ashutosh and Ramalingam, Chidambaram (2016) Bovine serum albumin interacts with silver nanoparticles with a ``side on'' or ``end on'' conformation. In: CHEMICO-BIOLOGICAL INTERACTIONS, 253 . pp. 100-111.

PDF Che_Bio_Int_253_100_2016.pdf - Published Version Restricted to Registered users only Download (1MB) | Request a copy

Abstract

As the nanoparticles (NPs) enter into the biological interface, they have to encounter immediate and first exposure to many proteins of different concentrations. The physicochemical interaction of NPs and proteins is greatly influenced not only by the number and type of proteins; but also the surface chemistry of NPs. To analyze the effects of NPs on proteins, the interaction between bovine serum albumin (BSA) and silver nanoparticles (AgNPs) at different concentrations were investigated. The interaction, BSA conformations, kinetics and adsorption were analyzed by UV-Visible spectrophotometer, dynamic light scattering (DLS), FT-IR spectroscopy and fluorescence quenching. DLS, FTIR and UV-visible spectrophotometric analysis confirms the interaction with minor alterations in size of the protein. Fluorescence quenching analysis confirms the side-on or end-on interaction of 1.5 molecules of BSA to AgNP. Further, pseudo-second order kinetics was determined with equilibrium contact-time of 30 min. The data of the present study determines the detailed evaluation of BSA adsorption on AgNP along with mechanism, kinetics and isotherm of the adsorption. (C) 2016 Elsevier Ireland Ltd. All rights reserved.

Item Type:	Journal Article
Publication:	CHEMICO-BIOLOGICAL INTERACTIONS
Publisher:	ELSEVIER IRELAND LTD
Additional Information:	Copy right for this article belongs to the ELSEVIER IRELAND LTD, ELSEVIER HOUSE, BROOKVALE PLAZA, EAST PARK SHANNON, CO, CLARE, 00000, IRELAND
Keywords:	Nanoparticle; Protein interaction; Langmuir; Order of reaction; Silver; Bovine serum albumin
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	05 Jul 2016 06:17
Last Modified:	05 Jul 2016 06:17
URI:	http://eprints.iisc.ac.in/id/eprint/54128

Actions (login required)

View Item