Formulation of nanotized curcumin and demonstration of its antimalarial efficacy

Ghosh, Aparajita and Banerjee, Tanushree and Bhandary, Suman and Surolia, Avadhesha (2014) Formulation of nanotized curcumin and demonstration of its antimalarial efficacy. In: INTERNATIONAL JOURNAL OF NANOMEDICINE, 9 . pp. 5373-5387.

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Abstract

Aim: The present study was conducted to overcome the disadvantages associated with the poor water solubility and low bioavailability of curcumin by synthesizing nanotized curcumin and demonstrating its efficacy in treating malaria. Materials and methods: Nanotized curcumin was prepared by a modified emulsion-diffusion-evaporation method and was characterized by means of transmission electron microscopy, atomic force microscopy, dynamic light scattering, Zetasizer, Fourier transform infrared spectroscopy, and differential thermal analysis. The novelty of the prepared nanoformulation lies in the fact that it was devoid of any polymeric matrices used in conventional carriers. The antimalarial efficacy of the prepared nanotized curcumin was then checked both in vitro and in vivo. Results: The nanopreparation was found to be non-toxic and had a particle size distribution of 20-50 nm along with improved aqueous dispersibility and an entrapment efficiency of 45%. Nanotized curcumin (half maximal inhibitory concentration IC50]: 0.5 mu M) was also found to be ten-fold more effective for growth inhibition of Plasmodium falciparum in vitro as compared to its native counterpart (IC50: 5 mu M). Oral bioavailability of nanotized curcumin was found to be superior to that of its native counterpart. Moreover, when Plasmodium berghei-infected mice were orally treated with nanotized curcumin, it prolonged their survival by more than 2 months with complete clearance of parasites in comparison to the untreated animals, which survived for 8 days only. Conclusion: Nanotized curcumin holds a considerable promise in therapeutics as demonstrated here for treating malaria as a test system.

Item Type:	Journal Article
Publication:	INTERNATIONAL JOURNAL OF NANOMEDICINE
Publisher:	DOVE MEDICAL PRESS LTD
Additional Information:	Copyright for this article belongs to the DOVE MEDICAL PRESS LTD, PO BOX 300-008, ALBANY, AUCKLAND 0752, NEW ZEALAND
Keywords:	curcumin; nanotized curcumin; P. berghei; P. falciparum; antimalarial
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	29 Dec 2014 05:40
Last Modified:	29 Dec 2014 05:40
URI:	http://eprints.iisc.ac.in/id/eprint/50547

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