X-ray structure of a galactose-specific lectin from Spatholobous parviflorous

Geethanandan, K and Abhilash, Joseph and Bharath, SR and Sadasivan, C and Haridas, M (2011) X-ray structure of a galactose-specific lectin from Spatholobous parviflorous. In: International Journal of Biological Macromolecules, 49 (5). pp. 992-998.

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Abstract

A galactose-specific seed lectin from Spatholobous parviflorus (SPL) has been purified, crystallized and its X-ray structure solved. It is the first lectin purified and crystallized from the genus Spatholobus (family: Fabaceae). The crystals belong to the space group P1, with a = 60.792 angstrom, b = 60.998 angstrom, c = 78.179 angstrom, alpha = 78.68 degrees, beta = 88.62 degrees, gamma = 104.32 degrees. The data were collected at 2.04 angstrom resolution under cryocondition, on a MAR image-plate detector system, mounted on a rotating anode X-ray generator. The coordinates of Dolichos biflorus lectin (1lu1) were successfully used for the structure solution by molecular replacement method. The primary structure of the SPL was not known earlier and it was unambiguously visible in the electron density. S. parviflorus lectin is a hetero-dimeric-tetramer with two alpha and two beta chains of 251 and 239 residues respectively. SPL has two metal ions, Ca(2+) and Mn(2+), bound to a loop region of each chain. The SPL monomers are in jelly roll form. (C) 2011 Elsevier B.V. All rights reserved.

Item Type:	Journal Article
Publication:	International Journal of Biological Macromolecules
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Spatholobous parviflorus;Jelly roll;Legume lectins; Haemagglutination;Molecular replacement
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	21 Dec 2011 09:14
Last Modified:	22 Feb 2012 07:17
URI:	http://eprints.iisc.ac.in/id/eprint/42594

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