Reddy, Bhanuprakash G and Bhat, Seetharam K (1998) Synergistic effect of UVB radiation and age on HMPS enzymes in rat lens homogenate. In: Journal of Photochemistry and Photobiology B: Biology, 43 (1). pp. 56-60.
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Abstract
The behaviour of rat lenticular enzymes, glucose-6-phosphate dehydrogena.se (G6PD, EC: 1.1.1.49) and 6-phosphogluconate dehydrogenase (6PGD, EC: 1.1.1.44) as a function of age and UVB irradiation (in vitro) was investigated by irradiating the lens homogenate from 3-and 12-month-old rats at 300 nm (100 μW cm 2). In the 3-month-old group the specific activities of G6PD and 6PGD were reduced by 26% and 42%, respectively, after 24 h of irradiation, whereas in the 12-month-old group the decrease was 38% and 49% respectively, which suggests that the susceptibility of HMPS enzymes to UVB damage is higher in older lenses. The decrease in specitic activity was associated with a change in apparent Km and Vmax (marginal in 3 months and significant in 12 months) of these enzymes due to UVB irradiation. UVB irradiation also decreased the levels of NADPH and NADPH/NADP ratio. These changes, altered activities of G6PD and 6PGD and altered levels of NADPH. may in turn have a bearing on lens transparency.
Item Type: | Journal Article |
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Publication: | Journal of Photochemistry and Photobiology B: Biology |
Publisher: | Elsevier Science |
Additional Information: | Copyright of this article belongs to Elsevier Science. |
Keywords: | Age;Enzymes;Lens;NADPH;Rat;UVB-radiation. |
Department/Centre: | Division of Biological Sciences > Molecular Biophysics Unit |
Date Deposited: | 12 Jul 2011 07:51 |
Last Modified: | 12 Jul 2011 07:51 |
URI: | http://eprints.iisc.ac.in/id/eprint/38775 |
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