Cloning and sequencing of winged bean (Psophocarpus tetragonolobus) basic agglutinin (WBA I): presence of second glycosylation site and its implications in quaternary structure

Sharma, Vivek and Srinivas, VR and Surolia, Avadhesha (1996) Cloning and sequencing of winged bean (Psophocarpus tetragonolobus) basic agglutinin (WBA I): presence of second glycosylation site and its implications in quaternary structure. In: FEBS Letters, 389 (3). pp. 289-292.

PDF http___www.sciencedirect.com_science__ob=MImg&_imagekey=B6T36-3Y0SK61-3G-2&_cdi=4938&_user=512776&_orig=search&_coverDate=07%2F08%2F1996&_sk=996109996&view=c&wchp=dGLbVzW-zSkzS&md5=af2a096fd86article.pdf - Published Version Restricted to Registered users only Download (435kB) | Request a copy

Abstract

We report cloning of the DNA encoding winged bean basic agglutinin (WBA I). Using oligonucleotide primers corresponding to N- and C-termini of the mature lectin, the complete coding sequence for WBA I could be amplified from genomic DNA. DNA sequence determination by the chain termination method revealed the absence of any intervening sequences in the gene. The DNA deduced amino acid sequence of WBA I displayed some differences with its primary structure established previously by chemical means. Comparison of the sequence of WBA I with that of other legume lectins highlighted several interesting features, including the existence of the largest specificity determining loop which might account for its oligosaccharide-binding specificity and the presence of an additional N-glycosylation site. These data also throw some light on the relationship between the primary structure of the protein and its probable mode of dimerization.

Item Type:	Journal Article
Publication:	FEBS Letters
Publisher:	Elsevier Science
Additional Information:	Copyright of this article belongs to Elsevier Science.
Keywords:	Primary structure;Legume lectin;PCR cloning;Sequence homology;Carbohydrate-binding site;N-linked glycosylation site.
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	20 Jan 2010 12:05
Last Modified:	19 Sep 2010 05:51
URI:	http://eprints.iisc.ac.in/id/eprint/24772

Actions (login required)

View Item