Krishna, R and Manjunath, GP and Kumar, P and Surolia, A and Nagasuma, Chandra R and Muniyappa, K and Vijayan, M (2006) Crystallographic identification of an ordered C-terminal domain and a second nucleotide-binding site in RecA: new insights into allostery. In: Nucleic Acids Research, 34 (9). 2186-2195.
PDF
3.pdf - Published Version Restricted to Registered users only Download (1MB) | Request a copy |
Abstract
RecA protein is a crucial and central component of the homologous recombination and DNA repair machinery. Despite numerous studies on the protein, several issues concerning its action, including the allosteric regulation mechanism have remained unclear. Here we report, for the first time, a crystal structure of a complex of Mycobacterium smegmatis RecA (MsRecA) with dATP, which exhibits a fully ordered C-terminal domain, with a second dATP molecule bound to it. ATP binding is an essential step for all activities of RecA, since it triggers the formation of active nucleoprotein filaments. In the crystal filament, dATP at the first site communicates with a dATP of the second site of an adjacent subunit, through conserved residues, suggesting a new route for allosteric regulation. In addition, subtle but definite changes observed in the orientation of the nucleotide at the first site and in the positions of the segment preceding loop L2 as well as in the segment 102-105 situated between the 2 nt, all appear to be concerted and suggestive of a biological role for the second bound nucleotide.
Item Type: | Journal Article |
---|---|
Publication: | Nucleic Acids Research |
Publisher: | Oxford University Press |
Additional Information: | Copyright of this article belongs to Oxford University Press. |
Department/Centre: | Division of Biological Sciences > Molecular Biophysics Unit Division of Interdisciplinary Sciences > Supercomputer Education & Research Centre Division of Biological Sciences > Biochemistry Division of Information Sciences (Doesn't exist now) > BioInformatics Centre |
Date Deposited: | 16 Mar 2009 10:38 |
Last Modified: | 19 Sep 2010 04:55 |
URI: | http://eprints.iisc.ac.in/id/eprint/17029 |
Actions (login required)
View Item |