Ghatpande, AS and Sikdar, SK (1999) Voltage-dependent gating of veratridine-modified RIIA Na+ channel \alpha subunit expressed heterologously in CHO cells. In: Pflugers Archiv European Journal of Physiology, 438 (3). pp. 378-383.
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Abstract
The voltage-dependent kinetics of veratridine-modified RIIA Na+ channel alpha subunit expressed heterologously in CHO cells were studied using the whole-cell patch-clamp technique. The activation and deactivation kinetics are well described by double exponential functions but poorly by a monoexponential function. Unlike the slow component, the fast time constant and associated amplitude factor depended steeply on the potential. The steady-state activation of veratridine-modified channels is described by a Boltzmann function with a V-1/2 of -131.9 mV and a slope of 9.41 mV. A two-state model is proposed for the fast component that explains the kinetics of veratridines mechanism of action.
Item Type: | Journal Article |
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Publication: | Pflugers Archiv European Journal of Physiology |
Publisher: | Springer Verlag |
Additional Information: | Copyright for this article belongs to Springer Verlag. |
Keywords: | kinetic analysis;patch clamp;RIIA sodium channel alpha subunit;veratridine induced modification |
Department/Centre: | Division of Biological Sciences > Molecular Biophysics Unit |
Date Deposited: | 04 Mar 2005 |
Last Modified: | 19 Sep 2010 04:15 |
URI: | http://eprints.iisc.ac.in/id/eprint/1538 |
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