Kinetic Analysis of the Binding of Ulex europeas Agglutinin 1 (UEA 1) to H-Antigenic Fucolipid

Thomas, Celestine J and Surolia, Avadhesha (2000) Kinetic Analysis of the Binding of Ulex europeas Agglutinin 1 (UEA 1) to H-Antigenic Fucolipid. In: Archives of Biochemistry and Biophysics, 374 (1). pp. 8-12.

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Abstract

The kinetics and thermodynamics of Ulex europeas agglutinin 1-H-antigenic determinant was studied by monitoring the binding of the lectin to H-antigenic fucolipid incorporated in liposomes tethered to the sensor surface by surface plasmon resonance. The second-order rate constants for the interactions are several order of magnitude slower than the diffusion controlled reactions. Analyses of activation parameters reveals that these slow second-order rate constants are a consequence of an unfavorable activation enthalpy term.

Item Type:	Journal Article
Publication:	Archives of Biochemistry and Biophysics
Publisher:	Academic Press
Additional Information:	Copyright of this article belongs to Academic Press
Keywords:	Biochemistry;Molecular Biophysics
Department/Centre:	Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited:	15 Oct 2007
Last Modified:	19 Sep 2010 04:39
URI:	http://eprints.iisc.ac.in/id/eprint/11984

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