Injectable biocompatible RAFT mediated nitroxide nanogels: A robust ROS-reduction antioxidant approach

Basak, S and Mukherjee, I and Das, TK (2024) Injectable biocompatible RAFT mediated nitroxide nanogels: A robust ROS-reduction antioxidant approach. In: Colloids and Surfaces B: Biointerfaces, 236 .

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Abstract

This work introduces novel nitroxide-based nanogels (NGs) crafted through controlled RAFT (Reversible Addition Fragmentation chain Transfer) polymerization, showcasing over 85 improved shelf-life compared to native superoxide dismutase (SOD) enzymes. These 30�40 nm NGs hold great promise for injectable delivery, effectively reducing foam cell formation and displaying potent antioxidant behavior against various reactive oxygen species (ROS), revolutionizing antioxidant therapy. Featuring a meticulously designed core-shell structure via precise RAFT polymerization, these NGs mimic SOD enzymatic activity with nitroxide-based antioxidants, providing unprecedented defense against ROS. Combining methacrylated 2,2,6,6-Tetramethyl-4-piperidyl methacrylate (PMA) and Glycidyl methacrylate (GMA) monomers with precisely synthesized nitroxyl radicals results in exceptional properties. Validated through comprehensive analytical methods, these NGs exhibit remarkable stability, halting foam cell formation even at high concentrations, and demonstrate notable biocompatibility. Their ability to protect low density lipoprotein (LDL) from oxidation for up to a month positions them at the forefront of combating cardiovascular diseases, especially atherosclerosis. This study pioneers injectable antioxidant therapy, offering an innovative approach to cardiovascular ailments. Targeting narrow plaques signifies a promising intervention, reshaping cardiovascular disease treatments. It highlights the potential of advanced drug delivery in biomedicine, promising more effective cardiovascular disease treatments. © 2024 Elsevier B.V.

Item Type:	Journal Article
Publication:	Colloids and Surfaces B: Biointerfaces
Publisher:	Elsevier B.V.
Additional Information:	The copyright for this article belongs to the Elsevier B.V. .
Keywords:	Acrylic monomers; Antioxidants; Biocompatibility; Cardiology; Controlled drug delivery; Diseases; Free radical polymerization; Living polymerization; Nanostructured materials; Oxygen; Superoxide dismutase; Targeted drug delivery, Enzyme mimetic nanogel; Enzyme mimetics; Injectables; Nanogels; Nitroxides; Reactive oxygen species; Reversible addition fragmentation chain transfer; Reversible addition fragmentation chain transfer nitroxide; Reversible addition-fragmentation chain transfer, Nanogels, 2,2,6,6 tetramethyl 4 piperidyl methacrylate; glycidylmethacrylate; low density lipoprotein; malonaldehyde; methacrylic acid derivative; nanogel; nitroxide nanogel; nitroxyl radical; radical; reactive oxygen metabolite; superoxide dismutase; unclassified drug, animal cell; antioxidant activity; Article; atherosclerosis; biocompatibility; cardiovascular disease; chemical modification; chemical reaction; chemical structure; concentration response; controlled study; cytotoxicity test; drug delivery system; enzyme activity; foam cell; lipid oxidation; molecular stability; mouse; nonhuman; oxidation; oxidative stress; particle size; polymerization; RAW 264.7 cell line; reversible addition fragmentation chain transfer; shelf life; synthesis; validation process; viscoelasticity
Department/Centre:	Division of Chemical Sciences > Inorganic & Physical Chemistry
Date Deposited:	23 Apr 2024 07:43
Last Modified:	23 Apr 2024 07:43
URI:	https://eprints.iisc.ac.in/id/eprint/84633

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