Kapoor, S and Adhikary, K and Kotak, S (2023) PP2A-B55SUR-6 promotes nuclear envelope breakdown in C. elegans embryos. In: Cell Reports, 42 (12).
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Abstract
Nuclear envelope (NE) disassembly during mitosis is critical to ensure faithful segregation of the genetic material. NE disassembly is a phosphorylation-dependent process wherein mitotic kinases hyper-phosphorylate lamina and nucleoporins to initiate nuclear envelope breakdown (NEBD). In this study, we uncover an unexpected role of the PP2A phosphatase B55SUR-6 in NEBD during the first embryonic division of Caenorhabditis elegans embryo. B55SUR-6 depletion delays NE permeabilization and stabilizes lamina and nucleoporins. As a result, the merging of parental genomes and chromosome segregation is impaired. NEBD defect upon B55SUR-6 depletion is not due to delayed mitotic onset or mislocalization of mitotic kinases. Importantly, we demonstrate that microtubule-dependent mechanical forces synergize with B55SUR-6 for efficient NEBD. Finally, our data suggest that the lamin LMN-1 is likely a bona fide target of PP2A-B55SUR-6. These findings establish a model highlighting biochemical crosstalk between kinases, PP2A-B55SUR-6 phosphatase, and microtubule-generated mechanical forces in timely NE dissolution. © 2023 The Author(s)
| Item Type: | Journal Article |
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| Publication: | Cell Reports |
| Publisher: | Elsevier B.V. |
| Additional Information: | The copyright for this article belongs to Author. |
| Keywords: | lamin, Article; Caenorhabditis elegans; cell cycle; cell nucleus membrane; microtubule; nonhuman; promoter region; protein targeting |
| Department/Centre: | Division of Biological Sciences > Microbiology & Cell Biology |
| Date Deposited: | 29 Feb 2024 05:41 |
| Last Modified: | 29 Feb 2024 05:41 |
| URI: | https://eprints.iisc.ac.in/id/eprint/83722 |
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