Supramolecular Interaction of Molecular Cage and β-Galactosidase: Application in Enzymatic Inhibition, Drug Delivery and Antimicrobial Activity

Mondal, A and Bhat, IA and Karunakaran, S and De, M (2021) Supramolecular Interaction of Molecular Cage and β-Galactosidase: Application in Enzymatic Inhibition, Drug Delivery and Antimicrobial Activity. In: ChemBioChem, 22 (11). pp. 1955-1960.

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Abstract

Enzyme inhibitors play a crucial role in diagnosis of a wide spectrum of diseases related to bacterial infections. We report here the effect of a water-soluble self-assembled PdII8 molecular cage towards β-galactosidase enzyme activity. The molecular cage is composed of a tetrapyridyl donor (L) and cis-[(en)Pd(NO3)2] (en=ethane-1,2-diamine) acceptor and it has a hydrophobic internal cavity. We have observed that the acceptor moiety mainly possesses the ability to inactivate the β-galactosidase enzyme activity. Kinetic investigation revealed the mixed mode of inhibition. This inhibition strategy was extended to control the growth of methicillin-resistant Staphylococcus aureus. The internalization of the Pd(II) cage inside the bacteria was confirmed when bacterial solutions were incubated with curcumin loaded cage. The intrinsic green fluorescence of curcumin made the bacteria glow when put under an optical microscope. Furthermore, this curcumin loaded molecular cage shows an enhanced antibacterial activity. Thus, PdII8 molecular cage is quite attractive due to its dual role as enzyme inhibitor and drug carrier.

Item Type:	Journal Article
Publication:	ChemBioChem
Publisher:	John Wiley and Sons Inc
Additional Information:	The copyright for this article belongs to John Wiley and Sons Inc.
Keywords:	beta galactosidase; curcumin; green fluorescent protein; palladium; antiinfective agent; beta galactosidase; coordination compound; enzyme inhibitor, antimicrobial activity; Article; bacterial cell; cell membrane permeability; chemical structure; concentration response; controlled study; drug delivery system; drug protein binding; drug synthesis; enzyme activity; enzyme inhibition; hydrophobicity; incubation time; methicillin resistant Staphylococcus aureus; microscopy; molecular interaction; nonhuman; chemical phenomena; chemistry; drug effect; macromolecule; metabolism; pharmacology; synthesis, Anti-Bacterial Agents; beta-Galactosidase; Coordination Complexes; Drug Delivery Systems; Enzyme Inhibitors; Hydrophobic and Hydrophilic Interactions; Macromolecular Substances; Methicillin-Resistant Staphylococcus aureus
Department/Centre:	Division of Chemical Sciences > Inorganic & Physical Chemistry Division of Chemical Sciences > Organic Chemistry
Date Deposited:	02 Mar 2023 09:37
Last Modified:	02 Mar 2023 09:37
URI:	https://eprints.iisc.ac.in/id/eprint/80828

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