Wishard, R and Jayaram, M and Ramesh, SR and Nongthomba, U (2023) Spatial and temporal requirement of Mlp60A isoforms during muscle development and function in Drosophila melanogaster. In: Experimental Cell Research, 422 (1).
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Abstract
Many myofibrillar proteins undergo isoform switching in a spatio-temporal manner during muscle development. The biological significance of the variants of several of these myofibrillar proteins remains elusive. One such myofibrillar protein, the Muscle LIM Protein (MLP), is a vital component of the Z-discs. In this paper, we show that one of the Drosophila MLP encoding genes, Mlp60A, gives rise to two isoforms: a short (279 bp, 10 kDa) and a long (1461 bp, 54 kDa) one. The short isoform is expressed throughout development, but the long isoform is adult-specific, being the dominant of the two isoforms in the indirect flight muscles (IFMs). A concomitant, muscle-specific knockdown of both isoforms leads to partial developmental lethality, with most of the surviving flies being flight defective. A global loss of both isoforms in a Mlp60A-null background also leads to developmental lethality, with muscle defects in the individuals that survive to the third instar larval stage. This lethality could be rescued partially by a muscle-specific overexpression of the short isoform. Genetic perturbation of only the long isoform, through a P-element insertion in the long isoform-specific coding sequence, leads to defective flight, in around 90 of the flies. This phenotype was completely rescued when the P-element insertion was precisely excised from the locus. Hence, our data show that the two Mlp60A isoforms are functionally specialized: the short isoform being essential for normal embryonic muscle development and the long isoform being necessary for normal adult flight muscle function. © 2022 Elsevier Inc.
| Item Type: | Journal Article |
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| Publication: | Experimental Cell Research |
| Publisher: | Elsevier Inc. |
| Additional Information: | The copyright for this article belongs to Elsevier. |
| Keywords: | Drosophila; Isoform switching; Muscle-LIM-Protein; Striated muscles; Z-discs |
| Department/Centre: | Division of Biological Sciences > Molecular Reproduction, Development & Genetics |
| Date Deposited: | 13 Jan 2023 09:54 |
| Last Modified: | 13 Jan 2023 09:54 |
| URI: | https://eprints.iisc.ac.in/id/eprint/79126 |
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