Intracellular trafficking and endocytic uptake pathway of Pepper vein banding virus-like particles in epithelial cells

Sabharwal, P and Amritha, CK and Sushmitha, C and Natraj, U and Savithri, HS (2019) Intracellular trafficking and endocytic uptake pathway of Pepper vein banding virus-like particles in epithelial cells. In: Nanomedicine, 14 (10). pp. 1247-1265.

PDF nan_14-10_1247-1265_2019.pdf - Published Version Restricted to Registered users only Download (9MB) | Request a copy

Abstract

Aim: Plant virus-like particles (VLPs) have emerged as a novel platform for delivery of drugs/antibodies. The aim of the present investigation is to establish the entry mechanism of flexuous rod-shaped virus particles into mammalian cells. Methods: Far-Western blot analysis, pull-down and ELISA were used to characterize vimentin and Hsp60 interaction with VLPs. The mode/kinetics of internalization of VLPs was deciphered using pharmacological inhibitors/endosomal markers. Results & discussion: The flexuous rod-shaped VLPs of Pepper vein banding virus (PVBV) enter HeLa and HepG2 cells via cell-surface proteins: vimentin and Hsp60, respectively. VLPs internalize via different modes of endocytosis in HeLa, HepG2 cells and are biodegradable. Vimentin and Hsp60 could be potential epithelial ligands that facilitate targeting of nanoparticles to tumor cells.

Item Type:	Journal Article
Publication:	Nanomedicine
Publisher:	Future Medicine Ltd.
Additional Information:	The copyright for this article belongs to Future Medicine Ltd.
Keywords:	chaperonin 60; vimentin; chaperonin 60; nanoparticle; vimentin, animal cell; Article; biodegradation; cell membrane; confocal microscopy; controlled study; endocytosis; endosome; enzyme linked immunosorbent assay; epithelium cell; flow cytometry; HeLa cell line; Hep-G2 cell line; human; human cell; internalization; intracellular transport; liquid chromatography-mass spectrometry; MDA-MB-231 cell line; molecular cloning; molecular imaging; mouse; NIH 3T3 cell line; nonhuman; Pepper vein banding virus; Potyvirus; priority journal; protein targeting; transmission electron microscopy; virus cell interaction; virus like agent; virus morphology; Western blotting; animal; cytoplasm; endocytosis; epithelium cell; kinetics; metabolism; physiology; Potyvirus; transport at the cellular level; virion; virus entry, Animals; Biological Transport; Chaperonin 60; Cytoplasm; Endocytosis; Epithelial Cells; HeLa Cells; Hep G2 Cells; Humans; Kinetics; Nanoparticles; Potyvirus; Vimentin; Virion; Virus Internalization
Department/Centre:	Division of Biological Sciences > Biochemistry
Date Deposited:	17 Nov 2022 05:50
Last Modified:	17 Nov 2022 05:50
URI:	https://eprints.iisc.ac.in/id/eprint/77960

Actions (login required)

View Item