Shahdeo, D and Chauhan, N and Majumdar, A and Ghosh, A and Gandhi, S (2022) Graphene-Based Field-Effect Transistor for Ultrasensitive Immunosensing of SARS-CoV-2 Spike S1 Antigen. In: ACS Applied Bio Materials, 5 (7). pp. 3563-3572.
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Abstract
Coronavirus disease (COVID-19) is an infectious disease that has posed a global health challenge caused by the SARS-CoV-2 virus. Early management and diagnosis of SARS-CoV-2 are crucial for the timely treatment, traceability, and reduction of viral spread. We have developed a rapid method using a Graphene-based Field-Effect Transistor (Gr-FET) for the ultrasensitive detection of SARS-CoV-2 Spike S1 antigen (S1-Ag). The in-house developed antispike S1 antibody (S1-Ab) was covalently immobilized on the surface of a carboxy functionalized graphene channel using carbodiimide chemistry. Ultraviolet-visible spectroscopy, Fourier-Transform Infrared Spectroscopy, X-ray Photoelectron Spectroscopy (XPS), Atomic Force Microscopy (AFM), Optical Microscopy, Raman Spectroscopy, Scanning Electron Microscopy (SEM), Enzyme-Linked Immunosorbent Assays (ELISA), and device stability studies were conducted to characterize the bioconjugation and fabrication process of Gr-FET. In addition, the electrical response of the device was evaluated by monitoring the change in resistance caused by Ag-Ab interaction in real time. For S1-Ag, our Gr-FET devices were tested in the range of 1 fM to 1 μM with a limit of detection of 10 fM in the standard buffer. The fabricated devices are highly sensitive, specific, and capable of detecting low levels of S1-Ag.
| Item Type: | Journal Article |
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| Publication: | ACS Applied Bio Materials |
| Publisher: | American Chemical Society |
| Additional Information: | The copyright for this article belongs to the Authors. |
| Keywords: | Antigens; Atomic force microscopy; Diagnosis; Diseases; Field effect transistors; Fourier transform infrared spectroscopy; Graphene; Graphene transistors; Scanning electron microscopy; Ultraviolet visible spectroscopy; X ray photoelectron spectroscopy, reductions; Coronaviruses; Field-effect transistor; Global health; Immunosensing; Infectious disease; MERS-CoV antigen; SARS-CoV-2 spike s1 antigen; Ultrasensitive; Viral spread, MERS, coronavirus spike glycoprotein; graphite; sarcoma-associated antigen S1; spike protein, SARS-CoV-2; tumor protein, chemistry; diagnosis; human, COVID-19; Graphite; Humans; Neoplasm Proteins; SARS-CoV-2; Spike Glycoprotein, Coronavirus |
| Department/Centre: | Division of Chemical Sciences > Solid State & Structural Chemistry Unit Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering Division of Physical & Mathematical Sciences > Physics |
| Date Deposited: | 10 Aug 2022 06:31 |
| Last Modified: | 10 Aug 2022 06:31 |
| URI: | https://eprints.iisc.ac.in/id/eprint/75802 |
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