Simultaneous multiple-level magnification selective plane illumination microscopy (sMx-SPIM) imaging system

S R, R and Chitre, K and Kurup, A and Nongthomba, U and Murty Srinivasula, S and Suheshkumar Singh, M (2022) Simultaneous multiple-level magnification selective plane illumination microscopy (sMx-SPIM) imaging system. In: Journal of Optics (United Kingdom), 24 (2).

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Abstract

We report an optical-based microscopy imaging technology-simultaneous multiple-level magnification selective plane illumination microscopy (sMx-SPIM) imaging system-that addresses a longstanding technological challenge of obtaining images, specifically of biological specimen non-destructively, at different field of views (FOVs) and spatial resolutions (or magnification powers) simultaneously in real-time. This imaging system provides not only 3D images but also time-resolved sequential images with temporal resolution ∼ msec. Magnification powers (or FOVs) of the individual images can be controlled independently that is achieved by housing two separate detection arms, in SPIM imaging system, fitted with objective lenses of different magnification powers. This unique feature holds promises to observe and study: (a) sub-microscopic details and entire structure of biological specimen side-by-side simultaneously and (b) spatio-temporal dynamics of functional activities of biological specimen. For validation study of robustness of the proposed sMx-SPIM imaging system, experiments are conducted in various biological samples including Danio rerio (zebrafish) embryo, Drosophila melanogaster, Allium cepa root, and A549 cell line. Experimental results demonstrate that the study is of significant impacts from two aspects, viz., technological implication and biological applications.

Item Type:	Journal Article
Publication:	Journal of Optics (United Kingdom)
Publisher:	IOP Publishing Ltd
Additional Information:	The copyright for this article belongs to the IOP Publishing Ltd.
Keywords:	Cell culture; Image resolution, 3d biological imaging; Biological imaging; Field of views; Light sheet microscopy or selective plane illumination microscopy; Light-sheet microscopies; Multiple field of view; Multiple level magnification; Multiple levels; Non destructive; Non-destructive biological imaging; Optical microscopes; Selective plane illuminations, Imaging systems
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics
Date Deposited:	27 Jun 2022 05:47
Last Modified:	27 Jun 2022 05:47
URI:	https://eprints.iisc.ac.in/id/eprint/73897

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