Fluorescence Signal Enhancement by a Spray-Assisted Layer-by-Layer Technique on Aluminum Tape Devices for Biosensing Applications

Boranna, R and Nataraj, CT and Bannur Nanjunda, S and Pahal, S and Jagannath, RK and Prashanth, GR (2022) Fluorescence Signal Enhancement by a Spray-Assisted Layer-by-Layer Technique on Aluminum Tape Devices for Biosensing Applications. In: Langmuir, 38 (10). pp. 3149-3157.

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Abstract

Layer-by-layer (LbL) self-assembled polyelectrolyte multilayer (PEM) films are a simple yet elegant bottom-up technology to create films at the nano-microscale. This low-cost technology has been widely used as a universal functionalization technique on a broad spectrum of substrates. Biomolecules under investigation can be incubated onto films based on complementary charge interactions between the films and biomolecules. There is a great demand for developing an ultralow-cost biosensing device, which can optimally enhance the fluorescence signal of the adsorbed biomolecules from the traditional labeled sensing platforms. In this work, we have incorporated a blend of the conventional metal enhanced fluorescence technology and the PEM as a dielectric spacer and functionalized film, coated on an aluminum paper (tape)-based substrate. These device has been found to be capable of holding biomolecules in three-dimensional PEM space. The devices fabricated by the proposed spray LbL technique provide significant fluorescence signal enhancement by holding a relatively higher mass per volume of the adsorbed biomolecules, when compared to traditional spin-and dip-coating techniques. Interestingly, our proposed device has expressed a fluorescence enhancement factor, which is 9 times higher than PEM-functionalized glass-based devices. To demonstrate the practical utility of our devices, we also compared our devices to Whatman FAST slides. Our experimental fluorescence results are almost comparable to Whatman FAST slides. Such PEM devices fabricated on top of low-cost aluminum tape using a spray LbL technique give new insights into the future development of ultralow-cost, high-throughput, and disposable lab-on-chip diagnostic applications.

Item Type:	Journal Article
Publication:	Langmuir
Publisher:	American Chemical Society
Additional Information:	The copyright for this article belongs to the American Chemical Society.
Keywords:	Aluminum; Aluminum coatings; Biomolecules; Costs; Multilayer films; Multilayers; Polyelectrolytes; Substrates, Biosensing applications; Fluorescence signals; Functionalized; Layer by layer; Layer-by-layer techniques; Polyelectrolyte multilayer; Polyelectrolyte multilayer film; Signal enhancement; Simple++; Whatman, Fluorescence, aluminum; polyelectrolyte, fluorescence, Aluminum; Fluorescence; Polyelectrolytes
Department/Centre:	Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Date Deposited:	01 Jul 2022 05:57
Last Modified:	01 Jul 2022 05:57
URI:	https://eprints.iisc.ac.in/id/eprint/73753

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