Aptamers for Viral Detection and Inhibition

Chakraborty, B and Das, S and Gupta, A and Xiong, Y and TV, V and Kizer, ME and Duan, J and Chandrasekaran, AR and Wang, X (2022) Aptamers for Viral Detection and Inhibition. In: ACS Infectious Diseases, 8 (4). pp. 667-692.

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Abstract

Recent times have experienced more than ever the impact of viral infections in humans. Viral infections are known to cause diseases not only in humans but also in plants and animals. Here, we have compiled the literature review of aptamers selected and used for detection and inhibition of viral infections in all three categories: humans, animals, and plants. This review gives an in-depth introduction to aptamers, different types of aptamer selection (SELEX) methodologies, the benefits of using aptamers over commonly used antibody-based strategies, and the structural and functional mechanism of aptasensors for viral detection and therapy. The review is organized based on the different characterization and read-out tools used to detect virus-aptasensor interactions with a detailed index of existing virus-targeting aptamers. Along with addressing recent developments, we also discuss a way forward with aptamers for DNA nanotechnology-based detection and treatment of viral diseases. Overall, this review will serve as a comprehensive resource for aptamer-based strategies in viral diagnostics and treatment.

Item Type:	Journal Article
Publication:	ACS Infectious Diseases
Publisher:	American Chemical Society
Additional Information:	The copyright for this article belongs to the American Chemical Society.
Keywords:	aptamer; DNA; genomic DNA; nanomaterial; nonstructural protein 5A; nonstructural protein 5B; protein; quantum dot, atomic force microscopy; capillary; capillary electrophoresis; capillary electrophoretic systematic evolution of ligands by exponential enrichment; cell systematic evolution of ligands by exponential enrichment; chemoluminescence; clinical trial (topic); colorimetry; comparative study; complex target systematic evolution of ligands by exponential enrichment; coronavirus disease 2019; DNA library; drug structure; electrochemical analysis; enzyme activity; enzyme inhibition; enzyme linked immunosorbent assay; fluorescence analysis; gene library; genome analysis; genomic systematic evolution of ligands by exponential enrichment; genomics; host cell; human; immunoaffinity chromatography; immunomagnetic separation; impedance; infection control; infectious agent; lateral flow immunochromatography; magnetic bead based microfluidic systematic evolution of ligands by exponential enrichment; microfluidic systematic evolution of ligands by exponential enrichment; microfluidics; nanotechnology; non electrochemical surface immobilized antibody; nonhuman; nucleotide sequence; optical sensor; protein targeting; Review; Severe acute respiratory syndrome coronavirus 2; sol-gel; structure analysis; surface enhanced Raman spectroscopy; surface plasmon resonance; systematic evolution of ligands by exponential enrichment aptamer technique; toggle systematic evolution of ligands by exponential enrichment; vaccination; virotherapy; virus attachment; virus detection; virus infection; virus inhibition; virus replication; voltammetry; whole cell
Department/Centre:	Division of Mechanical Sciences > Chemical Engineering
Date Deposited:	24 Jun 2022 11:49
Last Modified:	24 Jun 2022 11:49
URI:	https://eprints.iisc.ac.in/id/eprint/73692

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