Selvaraj, M and Singh, NS and Roy, Siddhartha and Sangeetha, R and Varshney, Umesh and Vijayan, M (2006) Cloning, expression, purification, crystallization and preliminary X-ray analysis of peptidyl-tRNA hydrolase from Mycobacterium tuberculosis. In: Acta Crystallographica Section F, 62 (9). pp. 913-915.
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Abstract
Peptidyl-tRNA hydrolase catalyses the cleavage of the ester link between the peptide and the tRNA in peptidyl-tRNAs that, for various reasons, have dropped off the translating ribosomes. This enzyme from Mycobacterium tuberculosis has been crystallized in three related but distinct forms: $P2_12_12_1$, unit-cell parameters a = 36.30, b = 61.85, c = 73.97 \AA , $P2_1$, a = 35.83, b = 73.79, c = 59.79 \AA ,$_\beta$ = 92.3, and $P2_12_12_1$, a = 35.84, b = 57.06, c = 72.59 \AA . X-ray data have been collected from all three forms.
Item Type: | Journal Article |
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Publication: | Acta Crystallographica Section F |
Publisher: | International Union of Crystallography |
Additional Information: | Copyright of this article belongs to International Union of Crystallography. |
Department/Centre: | Division of Biological Sciences > Molecular Biophysics Unit Division of Biological Sciences > Microbiology & Cell Biology |
Date Deposited: | 21 Nov 2006 |
Last Modified: | 19 Sep 2010 04:32 |
URI: | http://eprints.iisc.ac.in/id/eprint/8794 |
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