Anifowoshe, AT and Mukherjee, A and Ajisafe, VA and Raichur, AM and Nongthomba, U (2024) Synthesis and characterization of micro-sized polyisobutylene and evaluation of its toxicological effects on the development and homeostasis of zebrafish (Danio rerio). In: Scientific Reports, 14 (1).
|
PDF
Sci_rep_14_1_2024.pdf - Published Version Download (1MB) | Preview |
Abstract
Rampant industrialization has led to widespread reliance on hydrocarbon polymers for various commercial applications. While these synthetic polymers, commonly known as plastics, degrade in slowly in the environments, the toxic effects of their micro-sized particles remain underexplored. In this study, we synthesized polyisobutylene (PIB) microparticles in the lab and evaluated their toxicity and accumulation in a zebrafish model. Pristine and fluorescent PIB-microplastics (MPs), with particle sizes ranging from 2 to 10 μm, were synthesized using the solvent evaporation method. Fourier-transform infrared spectroscopy (FTIR) confirmed the stability of the suspensions. Zebrafish larvae exposed to various concentrations of PIB-MPs exhibited numerous morphological and molecular changes, including delayed hatching, impaired swimming behavior, increased reactive oxygen species levels, altered mRNA levels of genes encoding antioxidant proteins, and reduced survival rates. Dissections revealed PIB-MP accumulation in the guts of larvae and adult fish within 7�21 days, causing damage to the intestinal mucosa. These findings provide insights into how contaminants like PIB can induce pathophysiological defects in aquatic fauna and pose potential health hazards to humans. © The Author(s) 2024.
| Item Type: | Journal Article |
|---|---|
| Publication: | Scientific Reports |
| Publisher: | Nature Research |
| Additional Information: | The copyright for this article belongs to the author. |
| Keywords: | microplastic; polyene; polyisobutylene; polymer; reactive oxygen metabolite, animal; chemistry; drug effect; homeostasis; larva; metabolism; particle size; toxicity; water pollutant; zebra fish, Animals; Homeostasis; Larva; Microplastics; Particle Size; Polyenes; Polymers; Reactive Oxygen Species; Water Pollutants, Chemical; Zebrafish |
| Department/Centre: | Division of Biological Sciences > Molecular Reproduction, Development & Genetics Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy) |
| Date Deposited: | 25 Sep 2024 08:29 |
| Last Modified: | 25 Sep 2024 08:32 |
| URI: | http://eprints.iisc.ac.in/id/eprint/86245 |
Actions (login required)
 |
View Item |
