Lata, K and Charles, S and Mangala Prasad, V (2023) Advances in computational approaches to structure determination of alphaviruses and flaviviruses using cryo-electron microscopy. In: Journal of Structural Biology, 215 (3).
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Abstract
Advancements in the field of cryo-electron microscopy (cryo-EM) have greatly contributed to our current understanding of virus structures and life cycles. In this review, we discuss the application of single particle cryo-electron microscopy (EM) for the structure elucidation of small enveloped icosahedral viruses, namely, alpha- and flaviviruses. We focus on technical advances in cryo-EM data collection, image processing, three-dimensional reconstruction, and refinement strategies for obtaining high-resolution structures of these viruses. Each of these developments enabled new insights into the alpha- and flavivirus architecture, leading to a better understanding of their biology, pathogenesis, immune response, immunogen design, and therapeutic development. © 2023 Elsevier Inc.
| Item Type: | Journal Article |
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| Publication: | Journal of Structural Biology |
| Publisher: | Academic Press Inc. |
| Additional Information: | The copyright for this article belongs to authors. |
| Keywords: | antigen; epitope, Alphavirus; Article; clinical evaluation; computer model; cryoelectron microscopy; Flavivirus; human; image processing; immune response; maximum likelihood method; microbiological examination; motion correction algorithm; nonhuman; pathogenesis; receptor binding; signal noise ratio; three-dimensional imaging; transmission electron microscopy; two-dimensional imaging; virus morphology; Alphavirus; chemistry; cryoelectron microscopy; Flavivirus; procedures; virus, Alphavirus; Cryoelectron Microscopy; Flavivirus; Image Processing, Computer-Assisted; Viruses |
| Department/Centre: | Division of Biological Sciences > Molecular Biophysics Unit Division of Biological Sciences > Centre for Infectious Disease Research |
| Date Deposited: | 14 Dec 2024 19:33 |
| Last Modified: | 14 Dec 2024 19:33 |
| URI: | http://eprints.iisc.ac.in/id/eprint/85502 |
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