A LOW-COST PAPER-BASED SAMPLE PREPARATION MODULE TO LYSE BACTERIAL CELLS AND EXTRACT GENOMIC DNA USING ISOTACHOPHORESIS

Soni, S and Toley, BJ (2022) A LOW-COST PAPER-BASED SAMPLE PREPARATION MODULE TO LYSE BACTERIAL CELLS AND EXTRACT GENOMIC DNA USING ISOTACHOPHORESIS. In: MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences, 2022, pp. 101-102.

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Abstract

Point-of-care (POC) diagnostic system involving the nucleic acid amplification test (NAAT) has gained massive popularity because of its sensitivity and specificity. Very few of these tests can deal with crude biological samples. Therefore, most of these miniaturized POC devices often rely on expensive dedicated lab equipment for sample preparation, which involves isolating and purifying nucleic acids from the complex samples, such as blood, sputum, urine, or organic tissues. To overcome this limitation, we demonstrate a POC-compatible paper-based device capable of electrochemically lysis of bacterial (M. Smegmatis) cells along with purification and concentration of genomic DNA (gDNA) from viscous mock-sputum using isotachophoresis, within 20 min. This is the first demonstration of sputum DNA preparation using paper-based ITP. © 2022 MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences. All rights reserved.

Item Type:	Conference Paper
Publication:	MicroTAS 2022 - 26th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher:	Chemical and Biological Microsystems Society
Additional Information:	The copyright for this article belongs to Chemical and Biological Microsystems Society.
Keywords:	Costs; DNA; Electrophoresis; Genes, Bacterial lysis; Genomic DNA; Isotachophoresis; Low-costs; Nucleic acid amplification test; Nucleic acids amplification; Point of care; Point of care diagnostic; Sample preparation, Purification
Department/Centre:	Division of Mechanical Sciences > Chemical Engineering
Date Deposited:	15 Mar 2024 03:20
Last Modified:	15 Mar 2024 03:20
URI:	https://eprints.iisc.ac.in/id/eprint/84412

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