Augmenting Neutrophil Extracellular Traps with Carbonized Polymer Dots: A Potential Treatment for Bacterial Sepsis

Lin, C-J and Hwang, T-L and Wang, RYL and Nain, A and Shih, R-H and Chang, L and Lin, H-J and Harroun, SG and Chang, H-T and Huang, C-C (2024) Augmenting Neutrophil Extracellular Traps with Carbonized Polymer Dots: A Potential Treatment for Bacterial Sepsis. In: Small .

PDF sma_2024.pdf - Published Version Restricted to Registered users only Download (3MB) | Request a copy

Abstract

Sepsis is a life-threatening condition that can progress to septic shock as the body's extreme response to pathogenesis damages its own vital organs. Staphylococcus aureus (S. aureus) accounts for 50 of nosocomial infections, which are clinically treated with antibiotics. However, methicillin-resistant strains (MRSA) have emerged and can withstand harsh antibiotic treatment. To address this problem, curcumin (CCM) is employed to prepare carbonized polymer dots (CPDs) through mild pyrolysis. Contrary to curcumin, the as-formed CCM-CPDs are highly biocompatible and soluble in aqueous solution. Most importantly, the CCM-CPDs induce the release of neutrophil extracellular traps (NETs) from the neutrophils, which entrap and eliminate microbes. In an MRSA-induced septic mouse model, it is observed that CCM-CPDs efficiently suppress bacterial colonization. Moreover, the intrinsic antioxidative, anti-inflammatory, and anticoagulation activities resulting from the preserved functional groups of the precursor molecule on the CCM-CPDs prevent progression to severe sepsis. As a result, infected mice treated with CCM-CPDs show a significant decrease in mortality even through oral administration. Histological staining indicates negligible organ damage in the MRSA-infected mice treated with CCM-CPDs. It is believed that the in vivo studies presented herein demonstrate that multifunctional therapeutic CPDs hold great potential against life-threatening infectious diseases. © 2024 Wiley-VCH GmbH.

Item Type:	Journal Article
Publication:	Small
Publisher:	John Wiley and Sons Inc
Additional Information:	The copyright for this article belongs to John Wiley and Sons Inc.
Keywords:	Bacteria; Biocompatibility; Mammals, Antibiotics resistance; Bacterial sepsis; Carbonized polymer dot; Curcumin; Extracellular; Life threatening conditions; Methicillin; Neutrophile extracellular trap; Polymer dots; Resistant strains, Antibiotics
Department/Centre:	Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited:	01 Mar 2024 09:01
Last Modified:	01 Mar 2024 09:01
URI:	https://eprints.iisc.ac.in/id/eprint/83946

Actions (login required)

View Item