Integrating Live-Cell Imaging with Magnetic Manipulation System

Modak, P and Ramachandran, RV and Nahid, - and Bhat, R and Saini, DK and Ghosh, A (2023) Integrating Live-Cell Imaging with Magnetic Manipulation System. In: 6th International Conference on Manipulation, Automation, and Robotics at Small Scales, MARSS 2023, 9 - 13 October 2023, Abu Dhabi.

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Abstract

The internalization of magnetic nanorobots within live cells provides insights into their behaviour under the influence of an external magnetic field. However, the heat generated by the Helmholtz coil poses a significant challenge, leading to cell death. Most commercially available systems rely on metallic components to facilitate heat transfer. However, if these systems are placed inside the triaxial Helmholtz coil of the experimental setup, the following magnetic field will create eddy currents, leading to unintended heating consequences. Additionally, the limited space inside the Helmholtz coil presents a significant constraint for accommodating commercial systems. Integrating standard commercial live cell setups with magnetic propulsion setups, typically composed of triaxial Helmholtz, poses challenges. Additionally, thermal control is crucial as temperature maintenance within a degree is necessary, despite intermittent heat loads generated by coil drives with an extensive dynamic range. To address these issues, we have constructed and operated a system that successfully overcomes these challenges. A detailed account of the system's performance is given in this manuscript. The information presented herein will facilitate comprehensive biophysics studies. In this research, we present a unique setup that ensures precise temperature and pH control to enable extended viability of live cancer cells, facilitating in-depth studies of magnetic nanorobot behaviour within the cells subjected to an external magnetic field. The study of magnetic nanorobots within live cancer cells holds promise for advancing targeted therapies. However, maintaining appropriate temperature and pH conditions is critical for long-term cell viability. This research aims to overcome temperature fluctuations induced by the external magnetic field and incompatibility issues with commercially available systems by developing an integrated setup for optimal temperature and pH control. This innovative setup enables prolonged cell viability, facilitating in-depth investigations and advancing our understanding of magnetic nanorobot-cell interactions. © 2023 IEEE.

Item Type:	Conference Paper
Publication:	Proceedings of MARSS 2023 - 6th International Conference on Manipulation, Automation, and Robotics at Small Scales
Publisher:	Institute of Electrical and Electronics Engineers Inc.
Additional Information:	The copyright for this article belongs to Institute of Electrical and Electronics Engineers Inc.
Keywords:	Cancer cells; Diseases; Eddy currents; Heat transfer; Magnetic fields; Molecular biology; Nanorobotics; Nanorobots, Cell viability; External magnetic field; Helmholtz coil; Live cancer cells; Live cell; Live-cell imaging; Magnetic manipulation; Manipulation system; Nano robots; PH control, Cell death
Department/Centre:	Division of Biological Sciences > Molecular Reproduction, Development & Genetics Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering Division of Interdisciplinary Sciences > Centre for Nano Science and Engineering
Date Deposited:	29 Feb 2024 06:51
Last Modified:	29 Feb 2024 06:51
URI:	https://eprints.iisc.ac.in/id/eprint/83821

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