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PP2A-B55SUR-6 promotes nuclear envelope breakdown in C. elegans embryos

Kapoor, S and Adhikary, K and Kotak, S (2023) PP2A-B55SUR-6 promotes nuclear envelope breakdown in C. elegans embryos. In: Cell Reports, 42 (12).

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Official URL: https://doi.org/10.1016/j.celrep.2023.113495


Nuclear envelope (NE) disassembly during mitosis is critical to ensure faithful segregation of the genetic material. NE disassembly is a phosphorylation-dependent process wherein mitotic kinases hyper-phosphorylate lamina and nucleoporins to initiate nuclear envelope breakdown (NEBD). In this study, we uncover an unexpected role of the PP2A phosphatase B55SUR-6 in NEBD during the first embryonic division of Caenorhabditis elegans embryo. B55SUR-6 depletion delays NE permeabilization and stabilizes lamina and nucleoporins. As a result, the merging of parental genomes and chromosome segregation is impaired. NEBD defect upon B55SUR-6 depletion is not due to delayed mitotic onset or mislocalization of mitotic kinases. Importantly, we demonstrate that microtubule-dependent mechanical forces synergize with B55SUR-6 for efficient NEBD. Finally, our data suggest that the lamin LMN-1 is likely a bona fide target of PP2A-B55SUR-6. These findings establish a model highlighting biochemical crosstalk between kinases, PP2A-B55SUR-6 phosphatase, and microtubule-generated mechanical forces in timely NE dissolution. © 2023 The Author(s)

Item Type: Journal Article
Publication: Cell Reports
Publisher: Elsevier B.V.
Additional Information: The copyright for this article belongs to Author.
Keywords: lamin, Article; Caenorhabditis elegans; cell cycle; cell nucleus membrane; microtubule; nonhuman; promoter region; protein targeting
Department/Centre: Division of Biological Sciences > Microbiology & Cell Biology
Date Deposited: 29 Feb 2024 05:41
Last Modified: 29 Feb 2024 05:41
URI: https://eprints.iisc.ac.in/id/eprint/83722

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