Bio-mimicking the melanogenesis pathway using tyrosinase for dopamine sensing

Narasimhappa, P. and Ramamurthy, P.C. (2023) Bio-mimicking the melanogenesis pathway using tyrosinase for dopamine sensing. In: Electrochimica Acta, 469 .

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Abstract

An electrochemical sensor was fabricated using tyrosinase functionalized metal oxide nanoparticles that were used to detect dopamine (DA). The copper oxide nanoparticles (Cu2O NPs) were synthesized by hydrothermal method using Artemisia absinthium leaf extract. The surface morphology, optical characterization, and elemental composition of synthesized nanoparticles were characterized by various analytical instruments. The Line-weaver relationship was used to estimate the Km and Vmax values of the tyrosinase enzyme. Differential pulse voltammetry and cyclic voltammetry techniques were used to record the electrochemical responses of DA at Tyr/Cu2O NPs/GCE modified electrode. The modified electrode exhibits enhanced electro-catalytic activity towards DA more than the bare electrode that was supported by the lowering of critical voltage in the forward current-voltage characteristics. The Tyr/Cu2O NPs/GCE modified electrode exhibits a limit of detection (LOD) of 0.3 µM with a linear range of 10 µM to 70 µM concentration. Further study demonstrated that the modified electrochemical device had excellent repeatability and high selectivity to DA in the presence of various interference molecules. Analyses of real-world samples were demonstrated to estimate the practical applicability of Tyr/Cu2O NPs/GCE modified electrode. © 2023 Elsevier Ltd

Item Type:	Journal Article
Publication:	Electrochimica Acta
Publisher:	Elsevier Ltd
Additional Information:	The copyright for this article belongs to the Elsevier Ltd.
Keywords:	Catalyst activity; Copper oxides; Current voltage characteristics; Cyclic voltammetry; Electrochemical electrodes; Electrochemical sensors; Metal nanoparticles; Morphology; Neurophysiology; Surface morphology; Synthesis (chemical), Biomimicking; Copper oxide nanoparticles; Dopamine; Dopamine sensing; Functionalized; Melanogenesis; Metal oxide nanoparticles; Modified electrodes; Synthesised; Tyrosinase, Amines
Department/Centre:	Division of Interdisciplinary Sciences > Interdisciplinary Centre for Water Research Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited:	09 Nov 2023 11:23
Last Modified:	09 Nov 2023 11:23
URI:	https://eprints.iisc.ac.in/id/eprint/83301

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