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Deep proteome investigation of high-grade gliomas reveals heterogeneity driving differential metabolism of 5-aminolevulinic acid

Ghantasala, S and Bhat, A and Agarwal, U and Biswas, D and Bhattarai, P and Epari, S and Moiyadi, A and Srivastava, S (2023) Deep proteome investigation of high-grade gliomas reveals heterogeneity driving differential metabolism of 5-aminolevulinic acid. In: Neuro-Oncology Advances, 5 (1).

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Official URL: https://doi.org/10.1093/noajnl/vdad065


Background: Fluorescence-guided surgery (FGS) using 5-aminolevulinic acid (5-ALA) as adjunct for high-grade gliomas (HGGs) has been on the rise in recent years. Despite being largely effective, we observed multiple histologically similar sub-regions of the same tumor from a few individuals with varying protoporphyrin IX (PpIX) levels. The current study aims at understanding the proteomic changes driving differential metabolism of 5-ALA in HGGs. Methods: Biopsies were histologically and biochemically assayed. Following this, a deep proteomics investigation was carried out using high resolution liquid chromatography - mass spectrometry (HR LC-MS) to identify protein expression in differentially fluorescing regions of HGGs. Results: Our analysis identified 5437 proteins with high confidence. Differential analysis in the subgroup with HGGs carrying IDH mutation (IDH mt.) revealed 93 differentially regulated proteins (raw p-value ≤ 0.05 and absolute FC ≥ 1.5). Similar analysis in the IDH wild type (IDH wt.) subgroup revealed 20 differentially regulated proteins. Gene set enrichment analysis (GSEA) identified key pathways like ion channel transport, trafficking of AMPA receptors, and regulation of heme-oxygenase-1 in the IDH wt. subgroup. Pathways such as scavenging of heme, signaling by NOTCH4, negative regulation of PI3-AKT pathway, and iron uptake and transport were observed to be differentially regulated in the IDH mt. subgroup. Conclusions: Tumor regions from the same patient exhibiting differential fluorescence following 5-ALA administration were observed to have different proteome profiles. Future studies aimed at a better molecular understanding of 5-ALA metabolism in HGGs hold the potential to increase the efficacy of FGS and the use of 5-ALA as a theragnostic tool.

Item Type: Journal Article
Publication: Neuro-Oncology Advances
Publisher: Oxford University Press
Additional Information: The copyright for this article belongs to the Author.
Keywords: 5-ALA; deep proteome; GSEA; heme biosynthesis; HR LC-MS
Department/Centre: Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering
Date Deposited: 25 Jul 2023 06:06
Last Modified: 25 Jul 2023 06:06
URI: https://eprints.iisc.ac.in/id/eprint/82612

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