Singh, KK and Singh, DP and Singh, R and Saini, DK (2019) In vitro and in vivo Assessment of Protein Acetylation Status in Mycobacteria. In: Bio-protocol, 9 (13).
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Abstract
Protein acetylation is one of the standard post-translational modifications found in proteins across all organisms, along with phosphorylation which regulates diverse cellular processes. Acetylation of proteins can be enzymatically catalyzed through acetyltransferases, acetyl CoA synthetases or non-enzymatically through acyl carrier metabolic intermediates. In this protocol, using response regulator proteins as targets we describe the experimental strategy for probing the occurrence of acetylation using purified recombinant proteins in an in vitro setup. Further using M. smegmatis strains overexpressing the wild type or mutant response regulator protein, we also describe how in vivo acetylation can be validated in Mycobacterial proteins. The described approach can be used for analyzing acetylation of any mycobacterial protein under both in vitro and in vivo conditions. Copyright © 2019 The Authors; exclusive licensee Bio-protocol LLC.
| Item Type: | Journal Article |
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| Publication: | Bio-protocol |
| Publisher: | Bio-protocol LLC |
| Additional Information: | The copyright for this article belongs to the Authors. |
| Keywords: | Acetylation; KATms; M. tuberculosis; Post-translational modification; Response regulator; TcrX; Two-component signaling |
| Department/Centre: | Division of Biological Sciences > Molecular Reproduction, Development & Genetics Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering |
| Date Deposited: | 06 May 2023 03:20 |
| Last Modified: | 06 May 2023 03:20 |
| URI: | https://eprints.iisc.ac.in/id/eprint/81405 |
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