Sarkar, S and Goswami, D (2023) Lifetime of actin-dependent protein nanoclusters. In: Biophysical Journal, 122 (2). pp. 290-300.
|
PDF
bio_jou_122-2_290-300_2023.pdf - Published Version Download (2MB) | Preview |
Abstract
Protein nanoclusters (PNCs) are dynamic collections of a few proteins that spatially organize in nanometer-length clusters. PNCs are one of the principal forms of spatial organization of membrane proteins, and they have been shown or hypothesized to be important in various cellular processes, including cell signaling. PNCs show remarkable diversity in size, shape, and lifetime. In particular, the lifetime of PNCs can vary over a wide range of timescales. The diversity in size and shape can be explained by the interaction of the clustering proteins with the actin cytoskeleton or the lipid membrane, but very little is known about the processes that determine the lifetime of the nanoclusters. In this paper, using mathematical modeling of the cluster dynamics, we model the biophysical processes that determine the lifetime of actin-dependent PNCs. In particular, we investigated the role of actin aster fragmentation, which had been suggested to be a key determinant of the PNC lifetime, and we found that it is important only for a small class of PNCs. A simple extension of our model allowed us to investigate the kinetics of protein-ligand interaction near PNCs. We found an anomalous increase in the lifetime of ligands near PNCs, which agrees remarkably well with experimental data on RAS-RAF kinetics. In particular, analysis of the RAS-RAF data through our model provides falsifiable predictions and novel hypotheses that will not only shed light on the role of RAS-RAF kinetics in various cancers, but also will be useful in studying membrane protein clustering in general.
| Item Type: | Journal Article |
|---|---|
| Publication: | Biophysical Journal |
| Publisher: | Biophysical Society |
| Additional Information: | The copyright for this article belongs to the Authors. |
| Keywords: | MeSH Actins; Cytoskeleton; Membrane Proteins; Models, Theoretical; Signal Transduction EMTREE drug terms actin; membrane protein EMTREE medical terms cytoskeleton; signal transduction; theoretical model |
| Department/Centre: | Division of Physical & Mathematical Sciences > Physics |
| Date Deposited: | 09 Feb 2023 11:16 |
| Last Modified: | 09 Feb 2023 11:16 |
| URI: | https://eprints.iisc.ac.in/id/eprint/80105 |
Actions (login required)
 |
View Item |
