UHMWPE-MWCNT-nHA based hybrid trilayer nanobiocomposite: Processing approach, physical properties, stem/bone cell functionality, and blood compatibility

Naskar, S and Panda, AK and Jana, A and Kanagaraj, S and Basu, B (2020) UHMWPE-MWCNT-nHA based hybrid trilayer nanobiocomposite: Processing approach, physical properties, stem/bone cell functionality, and blood compatibility. In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, 108 (5). pp. 2320-2343.

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Abstract

The development of polymeric nanocomposites for biomedical applications remains a major challenge in terms of tailored addition of nanoparticles to realize the simultaneous enhancement of fracture resistance and cell/blood compatibility. To address this, the present work has been planned to determine whether small addition of surface functionalized multiwalled-carbon-nanotube, MWCNT (<1.5 wt) and egg-shell derived nanosized hydroxyapatite, nHA (<10 wt) to ultrahigh-molecular-weight-polyethylene (UHMWPE) can significantly improve the physical properties as well as biocompatibility. The difference in mouse osteoblast and human mesenchymal stem cell (hMSc) proliferation has been validated using both the monolithic composite and a trilayered composite with two different UHMWPE nanocomposites on either face with pure polymer at the middle. The combination of rheology and micro-CT with fractography reveals the homogeneous dispersion of nanofillers, leading to mechanical property enhancement. The quantitative analysis of cell viability and cell spreading by immunocytochemistry method, using vinculin and vimentin expression, establish significant cytocompatibility with hMSc and osteoblast cells onto the trilayer hybrid nanobiocomposite substrates. The hemocompatibility of the investigated composites under the controlled flow of rabbit blood in a microfluidic device reveals the signature of reduced thrombogenesis with reduction of platelet activation on UHMWPE nanocomposite w.r.t. unreinforced UHMWPE. An attempt has been made to discuss the blood compatibility results in the backdrop of the bovine serum albumin adsorption kinetics. Summarizing, the present study establishes that the twin requirement of mechanical property and cyto/hemo-compatibility can be potentially realized in developing trilayer composites in UHMWPE-nHA-MWCNT system.

Item Type:	Journal Article
Publication:	Journal of Biomedical Materials Research - Part B Applied Biomaterials
Publisher:	John Wiley and Sons Inc.
Additional Information:	The copyright for this article belongs to John Wiley and Sons Inc.
Keywords:	Biocompatibility; Biomechanics; Blood; Cell culture; Computerized tomography; Fracture mechanics; Functional polymers; Hydroxyapatite; Mammals; Mechanical properties; Medical applications; Microfluidics; Nanocomposites; Physical properties; Stem cells; Ultrahigh molecular weight polyethylenes, Bovine serum albumin adsorption; Cytocompatibility; Functionalized multi-walled carbon nanotubes; Hemocompatibility; Human mesenchymal stem cells; Nano-sized hydroxyapatite; Polymeric nanocomposites; Tri layers, Multiwalled carbon nanotubes (MWCN), bovine serum albumin; hydroxyapatite; multi walled nanotube; nanocomposite; ultra high molecular weight polyethylene; vimentin; vinculin; biomaterial; carbon nanotube; nanocomposite; polyethylene derivative; ultra-high molecular weight polyethylene; vimentin; vinculin, adsorption kinetics; adult; animal cell; Article; blood clotting; blood compatibility; bone cell; cell function; cell proliferation; cell spreading; cell viability; compression; contact angle; controlled study; egg shell; flow kinetics; human; human cell; immunocytochemistry; mesenchymal stem cell; micro-computed tomography; microfluidics; mouse; nonhuman; osteoblast; porosity; quantitative analysis; surface property; thrombocyte activation; wettability; X ray diffraction; Young modulus; Acetabularia; animal; cell adhesion; chemistry; cytology; Leporidae; materials testing; mesenchymal stem cell; metabolism; prosthesis implantation; thrombocyte; tissue engineering; tissue scaffold, Acetabularia; Animals; Biocompatible Materials; Blood Platelets; Cell Adhesion; Cell Proliferation; Durapatite; Humans; Materials Testing; Mesenchymal Stem Cells; Nanocomposites; Nanotubes, Carbon; Osteoblasts; Polyethylenes; Prosthesis Implantation; Rabbits; Rheology; Serum Albumin, Bovine; Tissue Engineering; Tissue Scaffolds; Vimentin; Vinculin; X-Ray Microtomography
Department/Centre:	Division of Chemical Sciences > Materials Research Centre Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering
Date Deposited:	06 Feb 2023 06:40
Last Modified:	06 Feb 2023 06:40
URI:	https://eprints.iisc.ac.in/id/eprint/79859

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