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Visualization of Single Dynein Molecules in Mammalian Cells

Tirumala, NA and Ananthanarayanan, V (2023) Visualization of Single Dynein Molecules in Mammalian Cells. In: Methods in molecular biology (Clifton, N.J.), 2623 . pp. 87-93.

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Official URL: https://doi.org/10.1007/978-1-0716-2958-1_6


In vitro single-molecule imaging experiments have provided insight into the stepping behavior, force production, and activation of several molecular motors. However, due to the difficulty in visualizing single molecules of motor proteins in vivo, the physiological function and regulation of motors at the single-molecule level have not been studied widely. Here, we describe how highly inclined and laminated optical sheet (HILO) microscopy can be adapted to visualize single molecules of the motor protein cytoplasmic dynein-1 in mammalian cells with high signal-to-noise ratio and temporal resolution. © 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Item Type: Journal Article
Publication: Methods in molecular biology (Clifton, N.J.)
Publisher: NLM (Medline)
Additional Information: The copyright for this article belongs to Humana Press Inc.
Keywords: cytoplasmic dynein; dynein adenosine triphosphatase, animal; mammal; mechanics; metabolism; microtubule; single molecule imaging, Animals; Cytoplasmic Dyneins; Dyneins; Kinesins; Mammals; Mechanical Phenomena; Microtubules; Single Molecule Imaging
Department/Centre: Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering
Date Deposited: 02 Feb 2023 10:28
Last Modified: 02 Feb 2023 10:28
URI: https://eprints.iisc.ac.in/id/eprint/79787

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