Silk composite interfacial layer eliminates rebleeding with chitosan-based hemostats

Indrakumar, S and Ghosh, S and Dash, TK and Mishra, V and Tandon, B and Chatterjee, K (2023) Silk composite interfacial layer eliminates rebleeding with chitosan-based hemostats. In: Carbohydrate Polymers, 304 .

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Abstract

Chitosan foams are among the approved hemostats for pre-hospital hemorrhagic control but suffer from drawbacks related to mucoadhesiveness and rebleeding. Herein, we have developed a designer bilayered hemostatic foam consisting of a bioactive layer composed of silica particles (≈300 nm) and silk fibroin to serve as the tissue interfacing component on a chitosan foam. The foam composition was optimized based on the in vitro clotting behavior and cytocompatibility of individual components. In vivo analysis in a rat model demonstrated that the developed hemostat could achieve rapid clotting (31 ± 4 s), similar to a chitosan-based hemostat, but the former had significantly lower blood loss. Notably, removal of the bilayered hemostat prevented rebleeding, unlike the chitosan foam, which was associated with markedly higher incidences of rebleeding (50 %) and left behind material residue. Thus, the designer bilayered foam presented here is a potent inducer of blood clotting whilst affording easy removal with minimal rebleeding.

Item Type:	Journal Article
Publication:	Carbohydrate Polymers
Publisher:	Elsevier Ltd
Additional Information:	The copyright for this article belongs to Elsevier Ltd.
Keywords:	Blood; Chitosan; Foams; Silica, Bilayered; Bilayered foam; Bioactive layers; Hemostat; In-vitro; Interfacial layer; Mucoadhesiveness; Non-adhesive; Silica particles; Silk fibroin, Adhesives
Department/Centre:	Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited:	02 Feb 2023 10:06
Last Modified:	02 Feb 2023 10:06
URI:	https://eprints.iisc.ac.in/id/eprint/79772

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