ePrints@IIScePrints@IISc Home | About | Browse | Latest Additions | Advanced Search | Contact | Help

Protein as evolvable functionally constrained amorphous matter

Tripathy, M and Srivastava, A and Sastry, S and Rao, M (2022) Protein as evolvable functionally constrained amorphous matter. In: Journal of Biosciences, 47 (4).

jou_bio_47-4_2022.pdf - Published Version

Download (1MB) | Preview
Official URL: https://doi.org/10.1007/s12038-022-00313-3


We explore current ideas around the representation of a protein as an amorphous material, in turn represented by an abstract graph G with edges weighted by elastic stiffnesses. By embedding this graph in physical space, we can map every graph to a spectrum of conformational fluctuations and responses (as a result of, say, ligand-binding). This sets up a ‘genotype–phenotype’ map, which we use to evolve the amorphous material to select for fitness. Using this, we study the emergence of allosteric interaction, hinge joint, crack formation and a slide bolt in functional proteins such as adenylate kinase, HSP90, calmodulin and GPCR proteins. We find that these emergent features are associated with specific geometries and mode spectra of floppy or liquid-like regions. Our analysis provides insight into understanding the architectural demands on a protein that enable a prescribed function and its stability to mutations. © 2022, Indian Academy of Sciences.

Item Type: Journal Article
Publication: Journal of Biosciences
Publisher: Springer
Additional Information: The copyright for this article belongs to Springer.
Keywords: Conformation fluctuations; fitness function; graph network; localized soft channels; protein allostery
Department/Centre: Division of Biological Sciences > Molecular Biophysics Unit
Date Deposited: 17 Jan 2023 10:03
Last Modified: 17 Jan 2023 10:03
URI: https://eprints.iisc.ac.in/id/eprint/79190

Actions (login required)

View Item View Item