Hyaluronic acid functionalized liposomes embedded in biodegradable beads for duo drugs delivery to oxaliplatin-resistant colon cancer

Tiwari, A and Gajbhiye, V and Jain, A and Verma, A and Shaikh, A and Salve, R and Jain, SK (2022) Hyaluronic acid functionalized liposomes embedded in biodegradable beads for duo drugs delivery to oxaliplatin-resistant colon cancer. In: Journal of Drug Delivery Science and Technology, 77 .

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Abstract

Background: Oxaliplatin (OHP) resistance is a major hurdle in the chemotherapeutic treatment of colorectal cancer (CRC). The present study aimed to formulate Eudragit S-100 (ES-100) coated alginate beads bearing drugs loaded targeted liposomes for simultaneous delivery of OHP and curcumin (CUR) to exert a synergistic therapeutic effect on OHP- resistant HT-29 cell line. Research design: The liposomes were prepared by the thin-film hydration method and optimized for various formulation parameters using a Box-Behnken design (BBD) with the aid of Design-Expert® software. Hyaluronic acid (HA) was conjugated on the liposomal surface using carbodiimide chemistry to target CD44 receptors, which are overexpressed on the CRC cells. HA coupled drugs bearing liposomes (OC-L-HA) were then characterized for various attributes, viz. shape, surface-morphology, size, zeta potential, PDI, entrapment efficiency, and drug loading, as well as in-vitro cell-based studies. OC-L-HA were entrapped in the alginate beads and examined for their in vitro potential and in vivo performances. Results: MTT assay demonstrated that OC-L-HA exhibited 2.76 and 2.58 fold higher cytotoxicity than targeted CUR liposomes and targeted OHP liposomes, respectively. The in vivo X-ray images affirmed the good targeting ability of the targeted beads to the colon. Conclusion: The outcomes of the studies revealed that these surface-modified liposomes entrapped in Eudragit S-100 coated beads could be an effective strategy for the treatment of CRC.

Item Type:	Journal Article
Publication:	Journal of Drug Delivery Science and Technology
Publisher:	Editions de Sante
Additional Information:	The copyright for this article belongs to Editions de Sante
Keywords:	Box-behnken design; Colorectal cancer; Curcumin; Eudragit S-100; Hyaluronic acid liposomes; Oxaliplatin
Department/Centre:	Division of Mechanical Sciences > Materials Engineering (formerly Metallurgy)
Date Deposited:	03 Jan 2023 05:01
Last Modified:	03 Jan 2023 05:01
URI:	https://eprints.iisc.ac.in/id/eprint/78665

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