Paddillaya, N and Mishra, A and Kondaiah, P and Pullarkat, P and Menon, GI and Gundiah, N (2019) Biophysics of Cell-Substrate Interactions Under Shear. In: Frontiers in Cell and Developmental Biology, 7 .
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Abstract
Cells adhere to substrates through mechanosensitive focal adhesion complexes. Measurements that probe how cells detach from substrates when they experience an applied force connect molecular-scale aspects of cell adhesion with the biophysical properties of adherent cells. Such forces can be applied through shear devices that flow fluid in a controlled manner across cells. The signaling pathways associated with focal adhesions, in particular those that involve integrins and receptor tyrosine kinases, are complex, receiving mechano-chemical feedback from the sensing of substrate stiffness as well as of external forces. This article reviews the signaling processes involved in mechanosensing and mechanotransduction during cell-substrate interactions, describing the role such signaling plays in cancer metastasis. We examine some recent progress in quantifying the strength of these interactions, describing a novel fluid shear device that allows for the visualization of the cell and its sub-cellular structures under a shear flow. We also summarize related results from a biophysical model for cellular de-adhesion induced by applied forces. Quantifying cell-substrate adhesions under shear should aid in the development of mechano-diagnostic techniques for diseases in which cell-adhesion is mis-regulated, such as cancers.
| Item Type: | Journal Article |
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| Publication: | Frontiers in Cell and Developmental Biology |
| Publisher: | Frontiers Media S.A. |
| Additional Information: | The copyright for this article belongs to the Authors. |
| Keywords: | biophysics; carcinoma cell; cell adhesion; cell interaction; human; Review; shear stress |
| Department/Centre: | Division of Biological Sciences > Molecular Reproduction, Development & Genetics Division of Interdisciplinary Sciences > Centre for Biosystems Science and Engineering Division of Mechanical Sciences > Mechanical Engineering |
| Date Deposited: | 11 Oct 2022 11:27 |
| Last Modified: | 11 Oct 2022 11:27 |
| URI: | https://eprints.iisc.ac.in/id/eprint/77417 |
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